Sökning: "Ellen Einarsson"

Hittade 2 uppsatser innehållade orden Ellen Einarsson.

1. 1. Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells

   Master-uppsats, Linköpings universitet/Teknisk biologi

   Författare :Ellen Einarsson; [2018]
   Nyckelord :Regulation; Untranslated regions; 5´ UTR; 3´ UTR; mRNA; Expression; Flow cytometry;

   Sammanfattning : The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein. LÄS MER

3. 2. Method Development for Determining the Stability of Heat Stable Proteins Combined with Biophysical Characterization of Human Calmodulin and the Disease Associated Variant D130G

   Kandidat-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

   Författare :Ehlimana Aleckovic; Linnea Andersson; Sherley Chamoun; Ellen Einarsson; Ebba Ekstedt; Emma Eriksen; Maria Madan-Andersson; [2016]
   Nyckelord :Calmodulin; wild type; D130G variant; method; affinity; trifluoperazine; p-HTMI.;

   Sammanfattning : Calmodulin is a highly conserved calcium ion binding protein expressed in all eukaryotic species. The 149 amino acid residues in the primary structure are organized in seven α helices with the highly flexible central α helix connecting the two non-cooperative domains of calmodulin. LÄS MER