Sökning: "Expressionssystem"
Visar resultat 1 - 5 av 9 uppsatser innehållade ordet Expressionssystem.
1. Functional Characterisation of Effectors from Biotrophic and Necrotrophic Wheat Pathogens in Heterologous Expression Systems
Master-uppsats, SLU/Dept. of Forest Mycology and Plant PathologySammanfattning : Effector proteins play an important role in the interactions between fungal pathogens and their host plant. Recognition of effectors can lead to localised cell death in plants. The necrotrophic fungus Parastagonospora nodorum utilises this mechanism to induce cell death in wheat and cause Stagonospora nodorum blotch (SNB). LÄS MER
2. Kartläggning av bioproduktion i Sverige : Behov, hinder och drivkrafter
Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildningSammanfattning : The market for drugs and treatments using biological products is rapidly growing. This is mainly due to the great potential that biological products have in comparison to traditional drugs. Biological products are composed of bigger molecules such as proteins and antibodies, and therefore allow a much more specific treatment. LÄS MER
3. Kloning, överuttryck och upprening av växtakvaporinet AtSIP1;1 i Escherichia coli
Kandidat-uppsats, Lunds universitet/Examensarbeten i molekylärbiologiSammanfattning : Heterologous overexpression of proteins signifies that the encoding gene of a protein in one organism is expressed in another host organism that normally does not have that gene in its genome. In basic biochemical research, this is an important method for studying various protein functions such as activity and interactions in biological processes, as well as obtaining sufficient amounts to determine protein structures. LÄS MER
4. Framtidens expressionssystem för svåruttryckta proteiner : Utvärdering av tolv expressionssystem
Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildningSammanfattning : Today, recombinant expression of proteins is used for a variety of purposes. One of these is the production of allergens, which are vital components in allergy diagnostics. However, traditional expression systems such as Escherichia coli and Pichia pastoris might not have the capacity to express all proteins of interest. LÄS MER
5. Production of gangliosidebiosynthetic membrane enzymes for biochemical and functionalstudies : Expression, purification and crystallizationoptimization of Thermococcus onnurineus Dolicho l-phosphate mannose synthase, Homosapiens and Branchiostoma floridae Glucosylceramide synthase
Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)Sammanfattning : Glycolipids play important roles in the biology of prokaryotes and eukaryotes, including humans, and although theyare found on the cell-membrane surface of all eukaryotic cells, not much is known about their biosynthesis. The aim ofthis project was to characterize two enzymes: glucosylceramide synthase (GCS) which is involved in the biosynthesisof glycolipids such as gangliosides that are abundant in the membranes of nerve cells; and dolicholphosphate mannosesynthase (DPMS), involved in the synthesis precursor for protein glycosylation. LÄS MER
