Sökning: "In situ Proximity Ligation Assay"

Hittade 4 uppsatser innehållade orden In situ Proximity Ligation Assay.

1. 1. Validation and optimization of multiplexInSitu PLA for signalling pathway analysis

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Tanay Kumar Sinha; [2021]
   Nyckelord :Chronic myeloid leukemia Tyrosine kinase inhibitors Proximity ligation assay Cross-reactivity Padlock Template;

   Sammanfattning : With the advent of Tyrosine kinase inhibitors (TKI) as a therapy for Chronic myeloid Leukemia (CML), the patients now enjoy a life expectancy close to that of the general population. But some patients do get unresponsive to the TKI treatment over time due to several mutations in the kinase domain of the BCR-ABL fusion protein, which further leads to activation of multiple signaling cascades within the leukemic cell, helping it survive and proliferate. LÄS MER

3. 2. Assay development for in situ detection of autophagy-related protein-protein interactions for characterization of colorectal cancer

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :M. Karoliina Hirvonen; [2015]
   Nyckelord :Autophagy; In situ Proximity Ligation Assay; Colorectal cancer;

   Sammanfattning : Every year, more than a million people are diagnosed with colorectal cancer (CRC) that develops in the large intestine. It is one of the most studied cancers in the world but still more knowledge about how this cancer develops and acts is needed in order to use more effective ways to treat CRC. LÄS MER

4. 3. Development of novel multiplexed systems for in situ PLA

   Master-uppsats, Institutionen för biologisk grundutbildning

   Författare :John Broberg; [2011]
   Nyckelord :Proximity ligation assay; PLA; Multiplex; Immunoassay; Rolling circle amplification; RCA; in situ; DNA sequence design;

   Sammanfattning : The in situ proximity ligation assay (in situ PLA) is an immunoassay that enables directvisualisation of single protein targets or protein interactions in cell or tissue samples. This project revolves around designing and introducing several novel multiplexable components tobe used in conjunction with Olink Bioscience's Duolink product line. LÄS MER

5. 4. Evaluation and development of reagents and improved protocol for flow cytometry readout using in situ PLA

   Uppsats för yrkesexamina på avancerad nivå, Institutionen för biologisk grundutbildning

   Författare :Sandra Ohlsson; [2011]
   Nyckelord :in situ proximity ligation assay; in situ PLA; flow cytometry; diagnostic method; cancer;

   Sammanfattning : The diagnosis of cancer today is obsolete, depending upon pattern recognition and non-quantifiable data. The time consuming diagnosis is often performed on biopsies, fixed using non standardised procedures, and leaves room for dubious results. LÄS MER