Sökning: "MTT assay"

Visar resultat 1 - 5 av 8 uppsatser innehållade orden MTT assay.

  1. 1. Studies of broad-spectrum inhibitors against main protease of SARS-CoV-2 and other Coronaviruses

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Alexandra Stanciu; [2023]
    Nyckelord :human coronavirus 229E; SARS-CoV-2; main protease; nirmatrelvir; GC-376; compound 19;

    Sammanfattning : Coronaviruses have caused three large outbreaks in the past century. The most recent one, also still ongoing, is represented by the SARS-CoV-2/Covid-19 pandemic. Efforts have been taken to develop efficient vaccines and antivirals and one of the major virus-based targets in drug development is represented by the main protease of these viruses. LÄS MER

  3. 2. Validation of in vitro cytotoxicity assays for cancer chemotherapy combining Celltiter Glo 2.0 assay with FMCA

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

    Författare :Mohanad Hajyahia; [2022]
    Nyckelord :Cellviability; Celltiter-Glo 2.0 reagent; fluorometric microculture cytotoxic assay; fluorescein diacetate; MTT assay and Survival Index SI % .;

    Sammanfattning : Background: Cancer is a common disease, and the choice of treatment becomes more difficult over time due to chemotherapy resistant in cancer cells. To improve the in vitroassay and the individual cancer treatment, a luminescence-based endpoint assay, CellTiter Glo 2. LÄS MER

  4. 3. Effects of skin care ingredients on keratinocytes : - Interplay between osmotic stress, cell viability, and gene expression towards increased understanding of keratinocyte differentiation

    Master-uppsats, Malmö universitet/Fakulteten för hälsa och samhälle (HS)

    Författare :Kassem Awad; [2021]
    Nyckelord :Cell viability; Keratinocytes; Osmotic stress; qPCR; Skin; Water activity;

    Sammanfattning : The epidermis is composed of multiple cell strata where viable keratinocytes, in the basal layer (stratum basale (SB)), go through a range of steps with the final stage of being dead corneocytes in the outer most layer (stratum corneum (SC)). The differentiation, which can be thought of as programmed cell death, include several key processes that are essential for an intact skin barrier. LÄS MER

  5. 4. Ouabain Toxicity -Selectivity Towards Renal Cancer Cells

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Emma Magnusson; [2020]
    Nyckelord :Cardiotonic steroids; ouabain; Na K -ATPase; cytotoxicity; renal cancer; apoptosis; Kardiotoniska steroider; ouabain; Na K -ATPase; cytotoxicitet; njurcancer; apoptos;

    Sammanfattning : Ouabain and other cardiotonic steroids are known to inhibit Na + ,K + -ATPase (NKA), theion pump responsible for the ionic gradient across the plasma membrane. These steroidsdisplay a selective toxicity towards several tumour cells in comparison to primary humancells, however, the mechanism behind this is not yet understood. LÄS MER

  7. 5. Protective capabilities of allopregnanolone against induced toxicity in SH-SY5Y cells relative to Alzheimer´s disease.

    Master-uppsats, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Mohamed Mustafa; [2020]
    Nyckelord :allopregnanolone; neurosteroid; alzheimer s disease; SH-SY5Y; neuroprotection;

    Sammanfattning : When the brain is exposed to a traumatic injury, the brain produces high amounts of neurosteroids like allopregnanolone and progesterone which show protective and neurogenic capacities. Alzheimer’s disease patients also have lower amounts of these neurosteroids in brain tissue. LÄS MER