Sökning: "Oligonukleotider"

Visar resultat 1 - 5 av 10 uppsatser innehållade ordet Oligonukleotider.

  1. 1. PAMPaR: Parallel Analysis of miRNAs, Proteins and RNAs. Dynamic Profiles of miRNAs, mRNAs and Specific Proteins in Mouse Embryonic Stem Cells Differentiating into Neural Precursors

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Amelie Carlström; [2023]
    Nyckelord :PAMPaR; SPARC; Proximity extension assay; Neuronal differentiation; Mouse embryonic stem cells; PAMPaR; SPARC; Proximity extension assay; Neuronal differentiering; embryonala musstamceller;

    Sammanfattning : MikroRNA-molekyler (miRNA) är viktiga icke-kodande RNA-molekyler för post-transkriptionell reglering av proteinkodande gener. De har även effekter på många cellulära processer såsom differentiering. De biosyntetiska reaktioner som skapar miRNA involverar proteiner som själva regleras av miRNA. LÄS MER

  3. 2. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

    Master-uppsats, Karlstads universitet

    Författare :Rebecka Jasinski; [2021]
    Nyckelord :oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

    Sammanfattning : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. LÄS MER

  4. 3. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    Kandidat-uppsats, Karlstads universitet

    Författare :Lewis Frazer; [2021]
    Nyckelord :Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Sammanfattning : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. LÄS MER

  5. 4. Functionalization and Evaluation of Nanoparticle Probes for the Development of a 14-Plex Diagnostic assay

    Master-uppsats, KTH/Kemi

    Författare :Samuel Narmack; [2021]
    Nyckelord :Functionalization; multiplex; probing; diagnostics; nanoparticles; Funktionalisering; multiplexera; sonder; diagnostik; nanopartiklar;

    Sammanfattning : Detta projekt var ett samarbete mellan Aplex Bio AB och Scilifelab. Projektets mål var att utveckla en molekylär diagnostisk panel med förmågan att detektera och diskriminera mellan 14 olika typer av patogener. Projektet innehåller 4 kapitel med fokus på olika mål. LÄS MER

  7. 5. Kombinationsmolekyler för genterapi : Konjugat mellan peptider och oligonukleotider

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Amanda Gustafsson; Vincent Goijenfalk; Frida Kasselstrand; Vendela Werner; Astrid Ulveström; Astrid Ekman; [2021]
    Nyckelord :;

    Sammanfattning : The report examines how the market, as well as purification conditions, forpeptide-oligonucleotide conjugates look like today. These conjugates are intended to be usedin drugs to treat otherwise incurable diseases but so far these types of molecules are still intest-phases and no finished conjugate is on the market yet. LÄS MER