Sökning: "Staphylococcal display"

Hittade 2 uppsatser innehållade orden Staphylococcal display.

1. 1. Bacterial Display of a Tau-Binding Affibody Construct:Towards Affinity Maturation

   Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Författare :Moira Ek; [2020]
   Nyckelord :Microtubule-associated protein tau; Neurodegenerative disorders; Alzheimer’s disease; Affibody molecules; Affinity maturation; Staphylococcal surface display; Escherichia coli display; Flow cytometry; Cell sorting;

   Sammanfattning : Aggregation of microtubule-associated protein tau is involved in the pathology of several neurodegenerative diseases, including Alzheimer’s disease. The affibody TP4 has been shown to inhibit this aggregation process, and its target-binding positions were previously grafted onto a dimeric affibody scaffold, creating the sequestrin seqTP4. LÄS MER

3. 2. Uttryck av cysteineproteaser HRV 3C, sortase A och TEV på ytan av prokaryota värdceller

   Master-uppsats, KTH/Skolan för bioteknologi (BIO)

   Författare :Therese Nilsson; [2015]
   Nyckelord :Cysteine proteases; E.coli display; FACS; HRV 3C; sortase A; Staphylococcal display; TEV;

   Sammanfattning : Proteases are important enzymes in the biotechnology due to their specific cleavage of substrates. HRV 3C, sortase A and TEV are some examples of cysteine proteases which become more of use lately in applications as removal of affinity tags (3C/TEV) and labelling of proteins (sortase). LÄS MER