Sökning: "metabolic engineering"

Visar resultat 1 - 5 av 37 uppsatser innehållade orden metabolic engineering.

  1. 1. Cloning and expressing the genes encoding Glycerol dehydratase (GDHt) and 1,3-Propanediol dehydrogenase (1,3-PDDH) in E. coli

    Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Författare :Eleni Pechlivani; [2023]
    Nyckelord :1; 3-Propanediol; Glycerol dehydratase; 3-Propanediol dehydrogenase; Glycerol dehydratase reactivase; E. coli; Protein expression; Biotechnology; Technology and Engineering;

    Sammanfattning : The biodiesel sector has witnessed tremendous growth in recent years, leading to a significant increase in glycerol production as a by-product. Addressing the challenge of glycerol valorization is crucial for the sustainability of the biodiesel industry. LÄS MER

  3. 2. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER

  4. 3. Novel tools for high-throughput genetic engineering, selection, and screening for improved Biotin production in E. coli cell factories

    Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Författare :Wibke Vellguth; [2023]
    Nyckelord :biotin; BioB; biotin synthase; oxidative stress; E. coli; biotechnology; Biology and Life Sciences;

    Sammanfattning : Biotin is an essential co-factor in various metabolic processes and is crucial for the growth and maintenance of living organisms. The market need for biotin is multifaceted and driven by its importance in human health, beauty and cosmetics applications, pharmaceuticals, animal nutrition, and other industries. LÄS MER

  5. 4. Prolongation of shelf life of minimally processed spinach by combining vacuum impregnation and electric field treatments

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Sophia Leffler; [2022]
    Nyckelord :spinach; pulsed electric field; moderate electric field; reversible electroporation; vacuum impregnation; shelf life; metabolic activity; respiration; food engineering; Agriculture and Food Sciences;

    Sammanfattning : A continuous growth of the world population raises the importance of preserving food resources while maintaining their nutrient value. Leafy vegetables such as spinach are a food category which decay quickly, and a large amount ends up as food waste. LÄS MER

  7. 5. Toward prototyping metabolic pathways in cyanobacteria using cell extracts

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Amina Bensabra; [2022]
    Nyckelord :cyanobacteria; metabolic engineering; cell-free metabolic engineering; enzymes; sustainability; cyanobakterier; metabolisk genteknik; syntetisk biologi; enzymer; hållbar bioproduktion;

    Sammanfattning : Cyanobakterier är intressanta mikroorganismer för produktion av biobränslen från solljus, vatten och atmosfärisk koldioxid och anses därför vara potentiella mikrobiella cellfabriker. Men på grund av långsam tillväxt och låg produktion är genteknologi processen intensiv och tidskrävande för cyanobakterier. LÄS MER