Metodvalidering av Cellient^TM genom jämförelse vid immuncytokemisk infärgning gentemot traditionell preparering av ascites- och pleuravätska

Sammanfattning:

When a patient is in urgent need of treatment, for a condition like cancer, a fast method to produce diagnosable material from the laboratory is desirable. Cellient^TM uses alcohol-fixation and enables diagnosis the same day the sample is received, while the standard method using formaldehyde takes over 48 hours before a diagnosis can be provided. Cellient^TM is fast and reduces human errors, but the affect it has on immunocytochemistry is not fully established. The purpose of this work was to validate Cellient^TM for clinical use. The validation was to establish whether cytological specimens prepared using the Cellient^TM gave comparable results to specimens prepared using the standard method. Specimens were prepared using both methods before immunocytochemical staining with antibodies was performed. Validation was performed using a parallel analysis of patient specimens using the Cellient^TM method and the standard method and then immunocytochemically staining the material with antibodies targeting CDX2, CK5, CK7, ER, Ki67, p63, TTF-1 and WT1. Staining was then compared between the methods for each specimen.

Because of an unsatisfactory number of specimens but also because of statistical differences between staining of the Cellient^TM material and the standard methods material the clinical use of the Cellient^TM could not be validated. The Cellient^TM provided a weaker staining in many cases. Thus further studies are needed which by optimizing the staining procedure might validate Cellient^TM for clinical use with ICC. An alternative would be to further utilize the already existing ThinPrep-method for ICC. It is desirable to leave methods involving hazardous formaldehyde.