Sökning: "5´ UTR"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden 5´ UTR.

1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

   Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

   Författare :Alexander Westlund; [2019]
   Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

   Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER

3. 2. Method for tracking orthogonal ribosomes in vivo using MS2coat protein

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Molekylärbiologi

   Författare :John Lindström; [2019]
   Nyckelord :;

   Sammanfattning : Ribosomes are large macromolecules responsible for protein synthesisand they consist of both RNA and proteins. Each ribosome is made of one large and one small subunit. Even though the ribosome is one of the most studied machineries in the cell there is a gap in our understanding of how this macromolecule functions in vivo. LÄS MER

4. 3. Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells

   Master-uppsats, Linköpings universitet/Teknisk biologi

   Författare :Ellen Einarsson; [2018]
   Nyckelord :Regulation; Untranslated regions; 5´ UTR; 3´ UTR; mRNA; Expression; Flow cytometry;

   Sammanfattning : The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein. LÄS MER

5. 4. Stabil och antibiotikafri läkemedelsproduktion i rekombinant Escherichia coli

   Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Kristina Benevides; Oscar Broström; Grim Elison Kalman; Hugo Swenson; Andrei Vlassov; Josefin Ågren; [2017]
   Nyckelord :copynumber; copy-number; copy; number; origin of replication; rekombinant; proteinexpression; läkemedel; E. coli; antibiotikafri; antibiotika; expressionssystem; expression; system; escherichia coli; kromosom; kromosombaserad; kromosomal; plasmidfri; plasmid; T7-promotor; T7; promotor; ompA; rrnB; autoinduktion; auto; induktion; matlab; kopietalmodell; utbytemodell; stabil; pH; pseudogen; pseudo; gen; caiB; yjjM; hsdS; yjiV; fermentor; batch; fed-batch; kontinuerlig; fed; batch; fermentation; Affibody; affibody-molekyl; affibody-molekyler; affibodymolekyl; affibodymolekyler; bioreaktor; acetat; acetatbildning; acetatproduktion; acetatreduktion; acetatreducering; peptidläkemedel; peptider; peptid; T7-system; operator; lac-operator; operon; 5 -UTR; 3 -UTR; sekundärstruktur; mRNA; T1; T2; terminator; termineringssekvens; optimering; lac-operon; repressor; inducering; BL21 DE3 ; BL21; B-stam; B; stam; modell; kopietal; kopie; tal; OriC;

   Sammanfattning : Den här rapporten presenterar ett antibiotikafritt, stabilt och kromosombaserat expressionssystem för läkemedelsproduktion i Escherichia coli på beställning av företaget Affibody AB. E. coli-stammen BL21(DE3) valdes som värdorganism för expressionssystemet. LÄS MER

7. 5. Analysis of conserved microRNA targets in the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster.

   Kandidat-uppsats, Institutionen för naturvetenskap, miljö och teknik

   Författare :Ninwa Youssef; [2013]
   Nyckelord :;

   Sammanfattning : MicroRNA (miRNA) is small regulatory non-coding single stranded RNA molecule that can repress protein expression either at transcriptional or translational level. Since their discoveries in nematodes in the early 1990´s extensive research have shown that this mechanism is conserved across species. LÄS MER