Sökning: "Affinity constants"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden Affinity constants.

  1. 1. The Role of Functional Traits and Trade-offs in Seasonal Succession of Phytoplankton Community Structuring : A numerical investigation of resource acquisition traits of Lake Constance

    Master-uppsats, Umeå universitet/Institutionen för ekologi, miljö och geovetenskap

    Författare :Ayesha Nagahage; [2024]
    Nyckelord :Phytoplankton; resource acquisition; traits; trade-off; resource limitation;

    Sammanfattning : Long-term ecological research in deep lakes offers valuable insights into understanding changes in trophic states and organization of phytoplankton assemblages. Utilizing five decades of phytoplankton taxonomic trait data from pre-alpine Lake Constance, a confirmed negative relationship was found between phosphate and light affinity at the annual community trait level. LÄS MER

  3. 2. Method Development for Determining the Stability of Heat Stable Proteins Combined with Biophysical Characterization of Human Calmodulin and the Disease Associated Variant D130G

    Kandidat-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Ehlimana Aleckovic; Linnea Andersson; Sherley Chamoun; Ellen Einarsson; Ebba Ekstedt; Emma Eriksen; Maria Madan-Andersson; [2016]
    Nyckelord :Calmodulin; wild type; D130G variant; method; affinity; trifluoperazine; p-HTMI.;

    Sammanfattning : Calmodulin is a highly conserved calcium ion binding protein expressed in all eukaryotic species. The 149 amino acid residues in the primary structure are organized in seven α helices with the highly flexible central α helix connecting the two non-cooperative domains of calmodulin. LÄS MER

  4. 3. Evaluation of Rate Constants from Protein-Ligand Interactions with Weak Affinity Chromatography

    Kandidat-uppsats, Institutionen för naturvetenskap, NV

    Författare :Daniel Jönsson; [2012]
    Nyckelord :peak profile method; dissociation association rate constants; weak affinity chromatography; band-broadening;

    Sammanfattning : The paradigm of drug discovery have been to find the strongest possible binder to the target by high-throughput screening (HTS) but high affinity interactions are related to low kinetic off rates and thus result in severe side-effects and non-approved drugs. Lead molecules working in a transient manner (KD > µM) will allow for rapid off rates and possibly less side-effects. LÄS MER

  5. 4. Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab

    Magister-uppsats, Institutionen för biologisk grundutbildning

    Författare :Fredrik Johansson; [2011]
    Nyckelord :Affinity; association rate constant; Bioaffy; characterization; Gyrolab; immunoassay; kinetics; microfluidics; monoclonal antibody; screening;

    Sammanfattning : The number of monoclonal antibodies approved for therapeutic use has increased rapidlyover the last decade. As a consequence, precise and robust kinetic characterization techniquesare crucial in order to select the best suitable candidates. A kinetic characterization methodwas developed in Gyrolab with automated sample transfers. LÄS MER

  7. 5. Affinity Determination of Protein A Domains to IgG subclasses by Surface Plasmon Resonance

    Magister-uppsats, Institutionen för fysik, kemi och biologi

    Författare :Sofia Nohldén; [2008]
    Nyckelord :Protein A; antibodies; IgG; SPR; Biacore; affinity determination;

    Sammanfattning : A capture step with protein A is the most common purification step in the downstream purification process of monoclonal antibodies. It is therefore of great importance to increase the knowledge of the interactions involved in this purification technique. LÄS MER