Sökning: "Allele-specific PCR"

Hittade 3 uppsatser innehållade orden Allele-specific PCR.

  1. 1. Exploring Swedish Oat (Avena sativa) Genetic History - from AD 1440 to today

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Amanda Raud Westberg; [2021]
    Nyckelord :;

    Sammanfattning : Oats (Avena sativa) have been one of the most important cereals in Swedish crop history, completely dominating domestic cereal production in the 19th and early 20th centuries. During this time, oats were mainly used as horse feed and since then, production has decreased with decreased use of horses. LÄS MER

  3. 2. Optimization of a multiplex ARMS-PCR for detection of the primary mutations causing Leber’s hereditary optic neuropath

    Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Författare :Klara Jäder; [2020]
    Nyckelord :Allele-specific PCR; LHON; Multiplex Polymerase Chain Reaction; mitochondrial DNA; single nucleotide polymorphism;

    Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is a genetic disease that causes the patients to become blind, first in one eye and then the other, around the ages of 10-75 years. The disease is caused by mutations in the mitochondrial DNA, which disturbs the respiratory chain leading to the deterioration of the retinal ganglion cells. LÄS MER

  4. 3. Optimization of a Multiplex PCR-RFLP Method Used for Detection of Three Primary Mutations in Leber’s Hereditary Optic Neuropathy Patients

    Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Författare :Emilia Nord; [2020]
    Nyckelord :LHON; Restriction enzyme; Multiplex PCR; Mutation detection; Mitochondrial mutation;

    Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is the most commonly inherited disease that causes blindness in one or both eyes, with a minimum prevalence of 1 in 31 000 in the northeast of England. What causes LHON is not fully known but three mitochondrial mutations, G3460A, G11778A, and T14484C, have been identified in over 95 % of all LHON patients. LÄS MER