Sökning: "Biacore"

Visar resultat 1 - 5 av 15 uppsatser innehållade ordet Biacore.

  1. 1. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Gustav Myhrinder; [2020]
    Nyckelord :Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Sammanfattning : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. LÄS MER

  2. 2. Interactions of the Human Recombinant Proteins JUNO and IZUMO1

    Kandidat-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Emma Lundell; [2020]
    Nyckelord :male infertility; surphace plasmon resonance; protein interactions; gold nanoparticles; protein immobilization; manlig infertilitet; ytplasmonresonans; proteininteraktioner; guldnanopartiklar; proteinimmobilisering;

    Sammanfattning : Det uppskattas att 15% av alla par världen över lider avinfertilitet. Ungefär hälften beror på manlig infertilitet och 40% av dessa fall kan ännu inte förklaras. Därmed är nuvarande metoder för att diagnostisera manlig infertilitet otillräckliga och ytterligare tekniker behövs. LÄS MER

  3. 3. Development of a label-free biosensor method for the identification of sticky compounds which disturb GPCR-assays

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Hamno Mohammed Kader; [2013]
    Nyckelord :Surface plasmon resonance SPR ; biosensor; Biacore; G-protein-coupled receptors GPCRs ; Low molecular weight LMW compounds; C-C chemokine receptor type 5 CCR5 ; acid-sensing ion channel 1a ASIC1a ; Thrombin; Carbonic Anhydrase II CA II ; P38α MAP kinase.;

    Sammanfattning : It is widely known that early estimates about the binding properties of drug candidates are important in the drug discovery process. Surface plasmon resonance (SPR) biosensors have become a standard tool for characterizing interactions between a great variety of biomolecules and it offers a unique opportunity to study binding activity. LÄS MER

  4. 4. A generic capture assay for immunogenicity, using Biacore

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Martin Engqvist; [2013]
    Nyckelord :Immunogenicity; immunoassay; capture assay; Biacore; non-specific binding; anti-drug antibodies ADA ; human plasma; anti-biotin; biotinylation;

    Sammanfattning : The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay. LÄS MER

  5. 5. Viktstudie av Analysinstrument : Konceptförslag för minskning av vikt

    M1-uppsats, Uppsala universitet/Institutionen för teknikvetenskaper

    Författare :Henrik Hultman; [2012]
    Nyckelord :Weight study; Viktstudie;

    Sammanfattning : This thesis report describes the development of concepts regarding how weight can be reduced to the analytical instrument Biacore 4000. This thesis has resulted in five different concept proposals, all of which is solutions to reduce the instrument's weight. LÄS MER