Sökning: "Biacore"

Visar resultat 6 - 10 av 18 uppsatser innehållade ordet Biacore.

  1. 6. Development of a label-free biosensor method for the identification of sticky compounds which disturb GPCR-assays

    Master-uppsats, Institutionen för biologisk grundutbildning

    Författare :Hamno Mohammed Kader; [2013]
    Nyckelord :Surface plasmon resonance SPR ; biosensor; Biacore; G-protein-coupled receptors GPCRs ; Low molecular weight LMW compounds; C-C chemokine receptor type 5 CCR5 ; acid-sensing ion channel 1a ASIC1a ; Thrombin; Carbonic Anhydrase II CA II ; P38α MAP kinase.;

    Sammanfattning : It is widely known that early estimates about the binding properties of drug candidates are important in the drug discovery process. Surface plasmon resonance (SPR) biosensors have become a standard tool for characterizing interactions between a great variety of biomolecules and it offers a unique opportunity to study binding activity. LÄS MER

  3. 7. A generic capture assay for immunogenicity, using Biacore

    Master-uppsats, Institutionen för biologisk grundutbildning

    Författare :Martin Engqvist; [2013]
    Nyckelord :Immunogenicity; immunoassay; capture assay; Biacore; non-specific binding; anti-drug antibodies ADA ; human plasma; anti-biotin; biotinylation;

    Sammanfattning : The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay. LÄS MER

  4. 8. Viktstudie av Analysinstrument : Konceptförslag för minskning av vikt

    M1-uppsats, Institutionen för teknikvetenskaper

    Författare :Henrik Hultman; [2012]
    Nyckelord :Weight study; Viktstudie;

    Sammanfattning : This thesis report describes the development of concepts regarding how weight can be reduced to the analytical instrument Biacore 4000. This thesis has resulted in five different concept proposals, all of which is solutions to reduce the instrument's weight. LÄS MER

  5. 9. SPR-based method for concentration determination of proteins in a complex environment

    Uppsats för yrkesexamina på avancerad nivå, Institutionen för biologisk grundutbildning

    Författare :Emma Ekström; [2012]
    Nyckelord :Protein concentration; surface plasmon resonance SPR ; Biacore; purification;

    Sammanfattning : In this project a method based on surface plasmon resonance has been developed for determining the concentration of several His-tagged proteins in complex solutions. It showed large dynamic range, no measureable non-specific binding and high sensitivity (with linear range around 0.1–10 μg/ml depending on the proteins). LÄS MER

  7. 10. New SPR based assays for plasma protein titer determination

    Master-uppsats, Institutionen för fysik, kemi och biologi

    Författare :Johan Kärnhall; [2011]
    Nyckelord :SPR; Surface Plasmon Resonance; Biacore; IgG; IgG Subclass; HSA; Albumin; plasma; plasma fractionation;

    Sammanfattning : Reliable analytical tools are important for time efficient and economical process development, production and batch release of pharmaceuticals. Therapeutics recovered from human plasma, called plasma protein products, involve a large pharmaceutical industry of plasma fractionation. LÄS MER