Sökning: "Cre-Lox recombination"

Hittade 3 uppsatser innehållade orden Cre-Lox recombination.

1. 1. Construction and Evaluation of a Cre-lox-Based Fluorescent Conjugation Tracking System

   Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Carl Brännström; [2022]
   Nyckelord :Antibiotic resistance; horizontal gene transfer; plasmids; conjugation; conjugational frequency; Cre loxP system;

   Sammanfattning : Plasmids are small, circular, extrachromosomal double-stranded genetic elements present in bacteria. Plasmids can replicate independently of the bacterial chromosome and play an important role as a transmitter of antibiotic resistance genes between bacteria. LÄS MER

3. 2. Construction and evaluation of a bacterial fluorescent conjugation tracking system

   Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Otto Lindahl; [2021]
   Nyckelord :;

   Sammanfattning : Plasmids are small, circular, extrachromosomal genetic elements that replicate independently of the bacterial chromosome. Plasmids play a crucial role in the dissemination of antibiotic resistance genes through conjugation. LÄS MER

4. 3. Optimization of PCR protocols used for genotyping transgenic mice & Evaluation of a method for co-detecting mRNA and protein

   Uppsats för yrkesexamina på grundnivå, Uppsala universitet/Jämförande fysiologi

   Författare :Amanda Isaksson; [2017]
   Nyckelord :Ventral tegmental area; Substantia nigra; Parkinson’s disease; transgenic mice; Cre-Lox recombination;

   Sammanfattning : The aim of the current study was divided into two separate goals, (i) optimization of a number of PCR-based protocols employed for genotyping transgenic mouse lines and (ii) evaluating a protocol for co-detection of mRNA and its correlated protein in the mouse midbrain. The optimization was performed on PCR protocols for genotyping the following transgenic mouse lines; Dat-Cre, Vglut2-Lox, Vglut2-Cre and Vmat2-Lox. LÄS MER