Sökning: "DNA isolation"

Visar resultat 1 - 5 av 28 uppsatser innehållade orden DNA isolation.

  1. 1. Expression of complement factor C1r and C1s in human gingival fibroblasts

    Master-uppsats, Umeå universitet/Institutionen för odontologi

    Författare :Hanna From; [2023]
    Nyckelord :fibroblasts; pEDS; C1r; C1s;

    Sammanfattning : Background: Periodontitis is an inflammatory condition rendering in degradation of tooth supporting tissue. In the inflammatory process cytokines, amongst others TNF-a, IL-1b and IL-6 play an important role in regulating the immune response. LÄS MER

  3. 2. Method development of magnetic cell isolation and DNA extraction of small cell populations from Ficoll-separated hematopoietic cells

    Kandidat-uppsats, Uppsala universitet/Institutionen för medicinsk cellbiologi

    Författare :Dominika Debowska; [2023]
    Nyckelord :Clonal haematopoiesis of indeterminate potential CHIP ; hematopoietic stem cell; automated cell separation; silica-membrane-based nucleic acid purification.;

    Sammanfattning : Clonal haematopoiesis of indeterminate potential, or CHIP are a family of mutations present in the general population. CHIP-mutations are prevalent in the haematopoietic stem cells and in the more mature cell populations, T-lymphocytes, B-lymphocytes and myeloid cells (CD3+, CD19+ and CD33+ cells) in blood. LÄS MER

  4. 3. Protoplast isolation and transformation in sweetpotato

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Jakob Steiner; [2022]
    Nyckelord :Protoplast; Sweetpotato; Isolation; Transformation; Regeneration; Callus; Propagation; In-vitro; Solanales; Celluase; Cell; Plant; Leaf; Petiole; Biotechnology; Biology and Life Sciences;

    Sammanfattning : Med målet att utvärdera en mindre restriktiv teknik för genmodifiering i sötpotatis (Ipomoea batatas), både gällande ekonomiska begränsningar såväl som det tid och energi bemödande som ett sådant projekt oundvikligen påbrår, har vi arbetat flitigt med att etablera optimerade parameters för protoplast isolation och fortsättningsvis också utforskat lovande protokoll för transformation och regeneration av dessa protoplaster, för att slutligen framställa nya genotyper. Således har en extensiv litteraturundersökning av tidigare publicerade protokoll, varav explantor från sötpotatis men också relaterade arter inom Solanales ordningen har diskuterats, utfärdades varefter datan analyserades via diverse avancerade statistiska modeller. LÄS MER

  5. 4. NLRP INFLAMMASOME : CRISPR knockout of RIPK3 gene in THP-1 monocytes

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Ryan Potter; [2020]
    Nyckelord :;

    Sammanfattning : Inflammation is a biological response to harmful stimuli which assists the overall immune response in defending the organism against potential threats like bacteria and other pathogens. This response is largely regulated by multiprotein complexes known as inflammasomes, which use one or more multi-stage networks to create a coordinated response across cell groups. LÄS MER

  7. 5. Isolation and genomic characterization of three PHA degrading bacteria from the marine environment

    Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Författare :Giulia Elli; [2019]
    Nyckelord :PHA; degradation of PHA; marine bacteria; sulphate reducing bacteria; SRB; SRM; biotechnology; bioteknik; Technology and Engineering;

    Sammanfattning : The broader goal of this project was to investigate what kind of effects the accumulation of polyhydroxyalkanoates (PHA) in the marine environment might have on the microbial world. Bacteria were isolated from biofilms fouling PHA pellets placed in the sediment-water interface; the goal was to isolate PHA degraders that were also sulphate reducers. LÄS MER