Sökning: "DNA restriction analysis"

Visar resultat 1 - 5 av 20 uppsatser innehållade orden DNA restriction analysis.

  1. 1. Uttryck av ett nytt rekombinant protein Cp149 (HBV-kapsidprotein) modifierat med TfR apical domän

    Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

    Författare :Hamida Noorzai; [2022]
    Nyckelord :Gene cloning; Recombinant DNA; Transformation; Protein expression; SDS-PAGE; Flow Cytometry; Genkloning; rekombinant DNA; Transformering; Proteinuttryck; SDS-PAGE; Flödescytometri;

    Sammanfattning : Hepatit-B är en leversjukdom som orsakas av hepatit-B-virus (HBV) vilket är en kapslad DNA-virus. Kapsidprotein (Cp) har stor betydelse i virusets livscykel exempelvis DNA-replikation, interaktion med värdceller och andra virala glykoproteiner. HBV, som många andra virus, tar sig in i cellen genom att binda till cellreceptorer. LÄS MER

  3. 2. Association between NT-proBNP rs198389-polymorphism and obstructive sleep apnea

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för hälsovetenskaper

    Författare :Vladyslav Melnyk; [2020]
    Nyckelord :;

    Sammanfattning : The Obstructive Sleep Apnea (OSA) disorder may lead to serious complications. The development of OSA is influenced in general by combination of both genetic and environmental factors. In particular, Single Nucleotide Polymorphisms (SNPs) were suggested to contribute to the development of OSA and its severity. LÄS MER

  4. 3. Genetic diversity status and associations with sperm quality characteristics in farmed Arctic charr (Salvelinus alpinus L.) across Sweden

    Master-uppsats, SLU/Dept. of Animal Breeding and Genetics

    Författare :Anam Anjum; [2020]
    Nyckelord :Arctic charr; dd-RADseq; genetic diversity; GWAS; reproductive success;

    Sammanfattning : The national breeding programme of Arctic charr (Salvelinus alpinus L.) has been of fundamental importance for the expansion of Arctic charr farming across Sweden. Selection has been ongoing since 1980s documenting approximately 10% improvement per generation regarding growth related traits. LÄS MER

  5. 4. Assessing the efficacy of artemisinin-based combination therapies (ACTs) against Plasmodium malariae and Plasmodium ovale infections with low parasite densities: overcoming challenges during molecular analyses

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Ioanna Broumou; [2020]
    Nyckelord :Malaria; Plasmodium malariae; Plasmodium ovale; low density infections; PCR detection;

    Sammanfattning : Background: Malaria is a major public health issue. Artemisinin-based combination therapies are the WHO recommended treatment for uncomplicated malaria. Plasmodium malariae and Plasmodium ovale infections are considered underestimated and the effectiveness of artemisinin-based combination treatments against them is poorly documented. LÄS MER

  7. 5. DNA based analyses of microbiota in fat fraction of raw milk

    Master-uppsats, SLU/Department of Molecular Sciences

    Författare :Caroline Blänning; [2017]
    Nyckelord :raw milk; microbiota; whole milk; DNA T-RFLP; PCR;

    Sammanfattning : The microbiota of raw milk is complex and the analysis methods have during the years developed from culturing to molecular based methods, allowing more precise descriptions of the composition. This project included both cultivation and molecular methods to compile the distri-bution of microorganisms in the whole milk, since the most common way to day to investigate milk microbiota is based on defatted milk. LÄS MER