Sökning: "DNA-polymerase"

Visar resultat 1 - 5 av 13 uppsatser innehållade ordet DNA-polymerase.

  1. 1. Understanding sequence errors in analysis of short tandem repeats through optimisation of a library preparation protocol utilising unique molecular indices

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Nelly Gyllö Lind; [2022]
    Nyckelord :polymerase chain reaction; massively parallel sequencing; DNA analysis; short tandem repeats; DNA polymerase; unique molecular indices;

    Sammanfattning : In the last few years massively parallel sequencing (MPS) has become important for forensic DNA analysis by providing new and broader possibilities, such as increased discrimination power for short tandem repeat (STR) analyses. An imperative part of MPS analysis is library preparation where there is a fundamental reliability on in vitro DNA polymerisation, which is prone to errors. LÄS MER

  3. 2. 3′-end fluorescence labelling of long RNAs using terminal deoxynucleotidyl transferase

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Johannes Lidberg; [2022]
    Nyckelord :Physical biochemistry; Fluorescence; In vitro transcription; RNA; Terminal deoxynucleotidyl transferase; Nucleobase analogue; Chemistry;

    Sammanfattning : Through recent years, RNA has rapidly gone from mainly being known as a passive information-carrying molecule within the central dogma of molecular biology to becoming the active ingredient in multiple therapeutics, such as in vaccines and potential drugs against cancer. However, one of the main challenges with RNA as a therapeutic is efficient delivery to cells. LÄS MER

  4. 3. Genetisk typning av ekvint herpesvirus typ 1 (EHV-1) : en studie av svenska stammar

    Uppsats för yrkesexamina på avancerad nivå, SLU/Dept. of Biomedical Sciences and Veterinary Public Health

    Författare :Johan Wilhelm Öhrmalm; [2022]
    Nyckelord :Ekvint herpesvirus 1; EHV-1; virusabort; PCR; sekvensering; fylogenetiskt träd;

    Sammanfattning : Ekvint herpesvirus typ 1 (EHV-1) är ett vanligt virus som infekterar hästar världen över. Viruset orsakar flera kliniska former av sjukdomen; feber, respiratorisk form, abort, neonatal död samt centralnervös form. I Sverige diagnosticeras omkring 10–30 utbrott per år på Statens veterinärmedicinska anstalt, SVA. LÄS MER

  5. 4. Generation of Doxycycline-Inducible Cell Lines Expressing Dominant-Negative DNA Polymerase γ and Mitochondrial Helicase Twinkle Variants

    Kandidat-uppsats, Umeå universitet/Biomedicinsk laboratorievetenskap

    Författare :Emil Nordeman; [2021]
    Nyckelord :mtDNA; Twinkle; Polymerase γ;

    Sammanfattning : .... LÄS MER

  7. 5. Development of a specific and sensitive method for detection and quantification of Ustilago nuda by qPCR

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Dambhare Setu; [2021]
    Nyckelord :Ustilago nuda; loose smut; real-time quantitative PCR; Seed-borne fungi; detection; probe;

    Sammanfattning : Loose smut of barley, caused by fungal pathogen Ustilago nuda is one of the major concerns throughout the globe for barley producers. The infection takes place without exhibiting any obvious symptoms and an infected seed lot can only be identified at the heading stage when the fungal teliospores emerge at the place of crop. LÄS MER