Sökning: "Elution"

Visar resultat 1 - 5 av 35 uppsatser innehållade ordet Elution.

  1. 1. Effects of phosphorylation on the ADP-ribosylation activity of PARP16

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Virginia Martín Cristóbal; [2023]
    Nyckelord :ADP-ribosylation; Kinases; PARP16; Phosphorylation; UPR biochemistry; Biochemistry; Chemistry;

    Sammanfattning : PARP16/ARTD15 is an intracellular mono-ADP-ribosyltransferase that catalyzes auto- and heteromodification by transfer of a single ADP-ribose moiety. It belongs to the ADP-ribosyltransferase (ART) superfamily. LÄS MER

  3. 2. Exploratory study of ion pair liquid chromatography separation methods and sample preparation procedures – for quantification of amount 5’capped and uncapped mRNA

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Felicia Lindström; [2023]
    Nyckelord :;

    Sammanfattning : Using ion pair chromatography, separation and analysis of shorter mRNA fragments was possible. The aim of the study was to develop a separation method for quantitation of 5’capped versus uncapped mRNA. Cleavage of mRNA was performed by complementary probes and the enzyme RNase H which created shorter mRNA fragments containing the 5’ end. LÄS MER

  4. 3. Investigation of Adsorption and Retention of Charged Compounds In RPLC

    Kandidat-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Emma Fryxelius; [2022]
    Nyckelord :adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

    Sammanfattning : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. LÄS MER

  5. 4. Unspecific interactions in different batch variations of agarose based base matrices at different NaCl concentrations.

    Master-uppsats, Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Författare :Kejvan Ghofranpanah; [2022]
    Nyckelord :Lysozyme; hydrophobicity; size exclusion chromatography; NaCl concentrations; isocratic elution; Lysozym; hydrofobicitet; size exclusion chromatography; NaCl-koncentrationer; isokratisk eluering;

    Sammanfattning : Size exclusion chromatography (SEC) separates molecules based on their actual size rather than their molecular weight. This indicates that the smaller a molecule is, the longer it will be held in the column since it can penetrate the pores more effectively than a bigger molecule, resulting in a longer retention time, whereas the larger molecule has a shorter retention time. LÄS MER

  7. 5. Evaluation of Pre-Analytical Processes on Lipemic Whole Blood Samples Used in Forensic Toxicology

    M1-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Emily Elenstål; [2022]
    Nyckelord :lipemia; post-mortal; whole blood; benzodiazepines; Intralipid; liquid-liquid extraction; solid-phase extraction;

    Sammanfattning : Introduction: Post-mortem whole blood samples differ greatly in quality, lipemia is one cause of concern in toxicological analyses. Around 4 % of all samples sent to RMV are given a notation of lipemic content. LÄS MER