Sökning: "Endo-T"

Hittade 3 uppsatser innehållade ordet Endo-T.

  1. 1. Structure-function study on the activation mechanism of Endo T : Crystallization and biochemical characterization of the intact Endo T from Trichoderma reesei (Hypocrea jecorina)

    Master-uppsats, SLU/Dept. of Molecular Biology (until 131231)

    Författare :Jonathan Boel; [2011]
    Nyckelord :Hypocrea jecorina Endo T; glycoside hydrolase family 18; EC 3.2.1.96; deglycosylation; proteolysis; C-terminus; protein structure;

    Sammanfattning : Endo T is a deglycosylating enzyme secreted by the filamentous fungus Trichoderma reesei (Hypocrea jecorina). The active Endo T protein is both deglycosylated and C-terminal processed. Since the intact protein was never observed in the T. reesei culture medium, a construct of the intact Endo T was expressed in P. LÄS MER

  3. 2. Mutational engineering, growth selection, expression, purification and crystallization of ligand bound endo-beta-N-acetylglucosaminidase T (Endo-T) from Hypocrea jecorina

    Magister-uppsats, SLU/Dept. of Molecular Biology (until 131231)

    Författare :Andreas Digre; [2010]
    Nyckelord :Endo-T; Endo-beta-N-acetylglucosaminidase; Deglycosylation; EC 3.2.1.96; Crystallography; Ligand bound structure; High mannose N-glycan; HPAEC-PAD;

    Sammanfattning : Recently, one of the first fungal-expressed, deglycosylating, Endo-beta-N-acetylglucosaminidases was found in the extracellular medium of soft-rot ascomycete Hypocrea jecorina (a.k.a. Trichoderma reesei) belonging to glycoside hydrolase family 18 (GH18). LÄS MER

  4. 3. Structure-function studies of biomass degrading enzymes : crystallization of EndoT from Hypocrea jecorina and Cel5H from Saccharophagus degradans, and enzyme hharacterization of Cel5H

    Master-uppsats, SLU/Dept. of Molecular Biology (until 131231)

    Författare :Jing Zhang; [2009]
    Nyckelord :Crystallography; EndoT; Enzyme characterization; HPLC; Endoglucanase activity;

    Sammanfattning : The purpose of this project was to investigate the structure-function relationship of two carbohydrate active enzymes, Endo-N-acetyl-β-(D)-glucosaminidase (EndoT) from Hypocrea jecorina and Cel5H from Saccharophagus degradans.The enzyme Endo-T from H. LÄS MER