Sökning: "Multiplex PCR"

Visar resultat 1 - 5 av 34 uppsatser innehållade orden Multiplex PCR.

  1. 1. Gonadal hypoplasia in Swedish Mountain cattle and other native Swedish cattle breeds

    Master-uppsats, SLU/Dept. of Animal Breeding and Genetics

    Författare :Julia Hinken; [2023]
    Nyckelord :gonadal hypoplasia; Swedish Mountain cattle; fjäll;

    Sammanfattning : The Swedish Mountain cattle and several related breeds show the phenotype of colour-sidedness. The genetic reasons for this phenotype are a translocation and duplication from chr6 to chr29 (known as Cs29) including the KIT gene and an additional translocation with part of the Cs29 translocation from chr29 back to chr6 (Cs6). LÄS MER

  3. 2. Utvärdering av BioFire Joint Infection Panel för mikrobiologisk diagnostik av ledvätska

    Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

    Författare :Tilda Bengtsson; [2023]
    Nyckelord :Synovial fluid; septic arthritis; multiplex PCR; FilmArray; BioFire Joint Infection Panel; Ledvätska; septisk artrit; multiplex PCR; FilmArray; BioFire Joint Infection Panel;

    Sammanfattning : En bakteriell ledinfektion kallas för septisk artrit och utan snabb behandling kan tillståndet leda till irreversibla ledskador och flera allvarliga komplikationer. Snabb diagnostik är viktigt för en god prognos och med nuvarande metod för odling av ledvätska tar det upp till fem dygn för detektion av mikroorganismer. LÄS MER

  4. 3. Activation of Placenta XBP-1 Signaling in Obese Women

    Kandidat-uppsats,

    Författare :Nabaa Ibraheem; [2021]
    Nyckelord :Western blot; placenta; fetma; graviditet; Multiplex qPCR; XBP-1 protein;

    Sammanfattning : Overweight (BMI 25–29) or obese (BMI over 30) pregnant women have an increased risk of complications during pregnancy and childbirth that can harm both women and their children.Due to increased risk for several complications such as of giving birth to large children, malformations or still births, the children have higher risk of obesity, blood pressure diseases and diabetes in childhood and later in life compared with children of normal weight. LÄS MER

  5. 4. Optimization of a multiplex ARMS-PCR for detection of the primary mutations causing Leber’s hereditary optic neuropath

    Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Författare :Klara Jäder; [2020]
    Nyckelord :Allele-specific PCR; LHON; Multiplex Polymerase Chain Reaction; mitochondrial DNA; single nucleotide polymorphism;

    Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is a genetic disease that causes the patients to become blind, first in one eye and then the other, around the ages of 10-75 years. The disease is caused by mutations in the mitochondrial DNA, which disturbs the respiratory chain leading to the deterioration of the retinal ganglion cells. LÄS MER

  7. 5. Optimization of a Multiplex PCR-RFLP Method Used for Detection of Three Primary Mutations in Leber’s Hereditary Optic Neuropathy Patients

    Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Författare :Emilia Nord; [2020]
    Nyckelord :LHON; Restriction enzyme; Multiplex PCR; Mutation detection; Mitochondrial mutation;

    Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is the most commonly inherited disease that causes blindness in one or both eyes, with a minimum prevalence of 1 in 31 000 in the northeast of England. What causes LHON is not fully known but three mitochondrial mutations, G3460A, G11778A, and T14484C, have been identified in over 95 % of all LHON patients. LÄS MER