Sökning: "Multiplex Polymerase Chain Reaction"

Visar resultat 1 - 5 av 10 uppsatser innehållade orden Multiplex Polymerase Chain Reaction.

1. 1. Utvärdering av BioFire Joint Infection Panel för mikrobiologisk diagnostik av ledvätska

   Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

   Författare :Tilda Bengtsson; [2023]
   Nyckelord :Synovial fluid; septic arthritis; multiplex PCR; FilmArray; BioFire Joint Infection Panel; Ledvätska; septisk artrit; multiplex PCR; FilmArray; BioFire Joint Infection Panel;

   Sammanfattning : En bakteriell ledinfektion kallas för septisk artrit och utan snabb behandling kan tillståndet leda till irreversibla ledskador och flera allvarliga komplikationer. Snabb diagnostik är viktigt för en god prognos och med nuvarande metod för odling av ledvätska tar det upp till fem dygn för detektion av mikroorganismer. LÄS MER

3. 2. Activation of Placenta XBP-1 Signaling in Obese Women

   Kandidat-uppsats,

   Författare :Nabaa Ibraheem; [2021]
   Nyckelord :Western blot; placenta; fetma; graviditet; Multiplex qPCR; XBP-1 protein;

   Sammanfattning : Overweight (BMI 25–29) or obese (BMI over 30) pregnant women have an increased risk of complications during pregnancy and childbirth that can harm both women and their children.Due to increased risk for several complications such as of giving birth to large children, malformations or still births, the children have higher risk of obesity, blood pressure diseases and diabetes in childhood and later in life compared with children of normal weight. LÄS MER

4. 3. Optimization of a multiplex ARMS-PCR for detection of the primary mutations causing Leber’s hereditary optic neuropath

   Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

   Författare :Klara Jäder; [2020]
   Nyckelord :Allele-specific PCR; LHON; Multiplex Polymerase Chain Reaction; mitochondrial DNA; single nucleotide polymorphism;

   Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is a genetic disease that causes the patients to become blind, first in one eye and then the other, around the ages of 10-75 years. The disease is caused by mutations in the mitochondrial DNA, which disturbs the respiratory chain leading to the deterioration of the retinal ganglion cells. LÄS MER

5. 4. Optimization of a Multiplex PCR-RFLP Method Used for Detection of Three Primary Mutations in Leber’s Hereditary Optic Neuropathy Patients

   Kandidat-uppsats, Uppsala universitet/Institutionen för kvinnors och barns hälsa

   Författare :Emilia Nord; [2020]
   Nyckelord :LHON; Restriction enzyme; Multiplex PCR; Mutation detection; Mitochondrial mutation;

   Sammanfattning : Leber’s hereditary optic neuropathy (LHON) is the most commonly inherited disease that causes blindness in one or both eyes, with a minimum prevalence of 1 in 31 000 in the northeast of England. What causes LHON is not fully known but three mitochondrial mutations, G3460A, G11778A, and T14484C, have been identified in over 95 % of all LHON patients. LÄS MER

7. 5. Detection of poleroviruses causing virus yellows in sugar beet

   Master-uppsats, SLU/Dept. Of Plant Biology

   Författare :Josefin Pettersson; [2020]
   Nyckelord :beet chlorosis virus; beet mild yellowing virus; sugar beet; Sweden; turnip yellows virus;

   Sammanfattning : Sugar beet is a crop cultivated for production of sugar. Many diseases can cause yield loss of sugar beet, where some are caused by viruses. Virus yellows is caused by several viruses and is visible as yellowing of sugar beet leaves. These viruses are transmitted by aphids and can be found across the world in temperate regions. LÄS MER