Sökning: "NGS"

Visar resultat 1 - 5 av 48 uppsatser innehållade ordet NGS.

  1. 1. Multiple-Emitter Super-resolution Imaging using the Alternating Descent Conditional Gradient Method

    Kandidat-uppsats, Lunds universitet/Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; Lunds universitet/Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation

    Författare :Dolev Illouz; [2023]
    Nyckelord :super-resolution; alternating descent conditional gradient method; ADCG; multiple-emitter localization; nanoscale imaging; single molecule tracking; Physics and Astronomy;

    Sammanfattning : This thesis examines the state-of-the-art 2D super-resolution technique alternating descent conditional gradient (ADCG) method's ability to accurately localize fluorophores in diffraction-limited single molecule images (SMI) and analyze the impact of pre-processing and post-processing modules on ADCG's fluorophore localization. A synthetic dataset obtained from the 2013 Grand Challenge localization microscopy and a temporally linked dataset obtained from an unpublished set of Optical DNA mapping experiments performed by Jonathan Jeffet at the NanoBioPhotonix Lab at Tel-Aviv University were initially segmented to extract their noise parameters. LÄS MER

  3. 2. EVALUATING TRANSCRIPTOME ASSEMBLY POTENTIAL BY DIFFERENT DE NOVO SEQUENCE ASSEMBLER TYPES

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Luan Gardenalli; [2023]
    Nyckelord :;

    Sammanfattning : With the rise of NGS technologies, the transcriptomes of non-model organisms can be reconstructed even with the absence of a reference genome, using de novo assembly tools. There is a wide range of de novo assembly tools frequently being developed, however, there is a still a knowledge gap about the different effects and efficiency of different de novo assembly software types for RNA-seq assembly. LÄS MER

  4. 3. PAMPaR: Parallel Analysis of miRNAs, Proteins and RNAs. Dynamic Profiles of miRNAs, mRNAs and Specific Proteins in Mouse Embryonic Stem Cells Differentiating into Neural Precursors

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Amelie Carlström; [2023]
    Nyckelord :PAMPaR; SPARC; Proximity extension assay; Neuronal differentiation; Mouse embryonic stem cells; PAMPaR; SPARC; Proximity extension assay; Neuronal differentiering; embryonala musstamceller;

    Sammanfattning : MikroRNA-molekyler (miRNA) är viktiga icke-kodande RNA-molekyler för post-transkriptionell reglering av proteinkodande gener. De har även effekter på många cellulära processer såsom differentiering. De biosyntetiska reaktioner som skapar miRNA involverar proteiner som själva regleras av miRNA. LÄS MER

  5. 4. A comparison between two computational tools estimating tumor purity using NGS data

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :My Eles; [2023]
    Nyckelord :;

    Sammanfattning : In 2020, cancer accounted for almost 20% of all deaths in the United States. Cancer is highly individual, and individualized treatments are essential in the battle against the disease. The tumor microenvironment is complex, and the cancer genome contains mutations driving the cancer. LÄS MER

  7. 5. Vilken ny kunskap ger aDNA-analyser om vegetationsutvecklingen i Nordeuropa under och efter Weichsel-istiden?

    Kandidat-uppsats, Lunds universitet/Geologiska institutionen

    Författare :Beatrix Cordes; [2023]
    Nyckelord :aDNA; ancient DNA; vegetationshistoria; vegetationsförändring; PCR; NGS; Nordeuropa; next generation sequencing; Weichselistiden; Earth and Environmental Sciences;

    Sammanfattning : Under de senaste 30 åren har en ny metod för att få information om tidigare ekosystem utveck- lats, nämligen att analysera gammalt (“ancient”) DNA (aDNA). Denna forskningsgren är fortfarande ung men har redan hittat många områden inom vilka den kan tillämpas. LÄS MER