Sökning: "Ni-NTA"

Hittade 5 uppsatser innehållade ordet Ni-NTA.

  1. 1. Effects of phosphorylation on the ADP-ribosylation activity of PARP16

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Virginia Martín Cristóbal; [2023]
    Nyckelord :ADP-ribosylation; Kinases; PARP16; Phosphorylation; UPR biochemistry; Biochemistry; Chemistry;

    Sammanfattning : PARP16/ARTD15 is an intracellular mono-ADP-ribosyltransferase that catalyzes auto- and heteromodification by transfer of a single ADP-ribose moiety. It belongs to the ADP-ribosyltransferase (ART) superfamily. LÄS MER

  3. 2. Characterization of Mitilysin Pores by Cryo-electron Microscopy

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Vladimir Novakovic; [2023]
    Nyckelord :Mitilysin; Mly; Structural Biology; Cryo-EM; pore; toxin; pore forming proteins; pore forming toxins; PFP; PFT;

    Sammanfattning : Pore forming toxins (PFTs) are a large group of proteins found mainly in bacteria with some exceptions found in animals. They bind and form pores in their target membranes and form pores, which leads to cell death. Among these are cholesterol-dependent cytolysins (CDC), which require the presence of cholesterol to bind target membranes. LÄS MER

  4. 3. IMAC - a review : The Establishment of a Small Challenger Company ina Segmented High-Technology Life Science Market

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Elisabeth Huss; Eriksson Malin; Henrik Sundqvist; [2016]
    Nyckelord :;

    Sammanfattning : This degree project report consists of an individual study, IMAC – a review, and a study made by a total of three students at the Uppsala School of Entrepreneurship, The Establishment of a Small Challenger Company in a Segmented High-Technology Life Science Market. The review is about immobilized metal ion affinity chromatography (IMAC) and describes this popular protein purification technique which has constantly evolved since its first discovery in 1975. LÄS MER

  5. 4. Production of recombinant keratinase for poultry feather degradation

    Magister-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Saeid Alinezhad; Amir Mirabdollah; [2009]
    Nyckelord :recombinant keratinase; bacillus megaterium; feathers degradation; expression vector;

    Sammanfattning : The keratinase gene (kerA) of Bacillus licheniformis ATCC®53757 was PCR amplified and subsequently cloned into Bacillus megaterium expression vector; pHIS1525.SPlipA and transformed in Bacillus megaterium ATCC®14945. The kerA gene carrying the recombinant plasmid pKERHIS1525. LÄS MER

  7. 5. Rye cell wall β-glucosidase: subcloning, expression and purification of recombinant protein from E.coli

    Uppsats för yrkesexamina på grundnivå, Institutionen för livsvetenskaper

    Författare :Nicolas Rochereau; [2007]
    Nyckelord :rye; defense system; β-glucosidase; subcloning; his-tag; thioredoxin-tag; E.coli; Ni-NTA; western blot;

    Sammanfattning : Several plant defense systems consist of enzymes that act on glucosides and produce a toxic compound. In the intact plant tissue the substrate and enzyme are kept apart. The system studied here consists of the substrate 2-O-β-D-glucopyranosyl-4-dihydroxy-1,4-benzoxazin-3-one and the enzyme glucan 1,3-β-glucosidase in rye. LÄS MER