Sökning: "Oligonucleotide"

Visar resultat 1 - 5 av 19 uppsatser innehållade ordet Oligonucleotide.

  1. 1. A novel assay for the detection of Hepatitis C virus in blood plasma, using padlock probes and rolling circle amplification

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Andréa Karlsson; [2023]
    Nyckelord :;

    Sammanfattning : Hepatitis C viral infection is a globally widespread blood-borne disease affecting the liver, causing cirrhosis and hepatocellular carcinoma. This type of liver cancer is mainly caused by chronic Hepatitis C and Hepatitis B. Specific detection with the following correct treatment is crucial to reduce the overall burden of the disease. LÄS MER

  3. 2. Signal amplification in a microfluidic immunoassay system via Binding Oligo Ladder Detection : Applying the Exazym® signal amplification to the Gyrolab® platform

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för farmaci

    Författare :Daniel Wiman; [2023]
    Nyckelord :immunoassay; ultra-sensitive; signal amplification; Gyrolab; Exazym; high sensitivity; microfluidics; BOLD amplification; nucleic acid polymerisation;

    Sammanfattning : Immunoassays are analytical methods that use the highly specific binding of antibodies in order to detect and quantify an analyte. The technique has become a staple in modern biopharmaceutical research and diagnostics, however the measurement of biomarkers like dysregulated cytokines require ultra-sensitive immunoassays that can detect molecules at sub pg/mL concentrations. LÄS MER

  4. 3. Non-coding RNAs as therapeutic agents : The future of therapy

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Hederoth Gustaf; Lyngå Olivia; Rudqvist Olivia; Jeppsson Rebecka; Hogolof Saida; Svanberg Sara; Kauppinen Veera; [2022]
    Nyckelord :Non-coding RNA; Oligonucleotide therapeutics;

    Sammanfattning : Non-coding RNA (ncRNA) therapeutics are based on short oligonucleotides, both naturally occurring and artificial, which target RNA in a site-specific way to modulate gene expression. As of today, 12 synthetically produced ncRNA-based drugs are available on the market in the US and Europe, and there is a possibility of more to be approved in the near future. LÄS MER

  5. 4. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    Kandidat-uppsats, Karlstads universitet

    Författare :Lewis Frazer; [2021]
    Nyckelord :Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Sammanfattning : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. LÄS MER

  7. 5. Kombinationsmolekyler för genterapi : Konjugat mellan peptider och oligonukleotider

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Amanda Gustafsson; Vincent Goijenfalk; Frida Kasselstrand; Vendela Werner; Astrid Ulveström; Astrid Ekman; [2021]
    Nyckelord :;

    Sammanfattning : The report examines how the market, as well as purification conditions, forpeptide-oligonucleotide conjugates look like today. These conjugates are intended to be usedin drugs to treat otherwise incurable diseases but so far these types of molecules are still intest-phases and no finished conjugate is on the market yet. LÄS MER