Sökning: "Plasmid"

Visar resultat 1 - 5 av 56 uppsatser innehållade ordet Plasmid.

  1. 1. Investigating the function of GroES with hard-to-fold proteins in vivo

    L3-uppsats, Linköpings universitet/KemiLinköpings universitet/Tekniska fakulteten; Linköpings universitet/Institutionen för klinisk och experimentell medicinLinköpings universitet/Medicinska fakulteten

    Författare :Oliver Hild Walett; Emmanuel Berlin; Johan Larsson; Sofie Arvidsson; Filip Fors; Marianne Gavelius; Filip Genander; Johanna Granqvist; Philip Lifwergren; Alexandra Sandéhn; Martin Viksten; Irma Wenhov; [2019]
    Nyckelord :;

    Sammanfattning : The use of molecular chaperones can increase the yield of correctly folded proteins. This is especially needed in the expression of proteins non-native to the host organism. This study set out to investigate the function of the chaperone GroES; a component in the GroE-system. The function of this chaperone has only been studied alone in vitro. LÄS MER

  2. 2. Antibiotic-Regulated Plasmid Copy Number Variation: A Driver of Antibiotic Resistance?

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Ahmed Eldek; [2019]
    Nyckelord :antibiotic resistance; plasmid copy number; ddPCR;

    Sammanfattning : Plasmids are small circular DNA molecules within bacterial cells that are separated from the bacterial chromosome and replicate independently. Also, they play a crucial role in the dissemination of antibiotic resistance genes among bacteria through horizontal gene transfer. LÄS MER

  3. 3. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Alexander Westlund; [2019]
    Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER

  4. 4. Finding a stabilized sequence for reconstructed Icosahedra Lumazine Synthase by random mutagenesis and Fluorescence-Activated Cell Sorting

    Master-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Caroline Olsson; [2019]
    Nyckelord :Icosahedra; Assembly; Lumazine Synthase; Fluorescence-Activated Cell Sorting; FACS; Directed evolution; Random mutagenesis; Stability; Structure; Biochemistry; Biokemi; Chemistry; Biology and Life Sciences;

    Sammanfattning : Lumazine Synthase forms pentamers, C5 in some organism and icodahedra, Ih in other. The aim of this project is to investigate which evolutionary steps could have led the pentamers to assembly into icosahedral capsids. LÄS MER

  5. 5. A novel Adenoviral miRNA, a candidate for development of a novel gene therapy startegy

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Benjamin Danish; [2019]
    Nyckelord :A novel Adenoviral miRNA;

    Sammanfattning : In 2017, a novel miRNA was found at the MLTU-region of adenoviral genome, termed as MLP-TSS-sRNA. This current study started with performing a series of mutations in the MLP-TSS-sRNA in order to investigate how the MLP-TSS-sRNA as a single stranded small RNA was protected from rapid RNA degradation in transfected cells (in vivo). LÄS MER