Sökning: "Plasmid"

Visar resultat 21 - 25 av 93 uppsatser innehållade ordet Plasmid.

  1. 21. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Olivia Alexandra; [2021]
    Nyckelord :Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

    Sammanfattning : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. LÄS MER

  3. 22. The use of a CRISPR-Cas9 system to protect probiotic strains from transferrable drug resistance genes

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Sara Lundberg; [2021]
    Nyckelord :CRISPR-Cas9; antibiotic resistance; horizontal gene transfer; probiotic bacteria;

    Sammanfattning : The discovery of antibiotics have revolutionized modern medicine, facilitating the treatment of a variety of bacterial infections, enabled surgeries otherwise impossible to perform and increased life expectancy in all countries. However, the rapid development of resistance among microorganisms and the increasing numbers of clinical outbreaks caused by multiresistant bacteria have accelerated the need for new alternatives to antibiotics. LÄS MER

  4. 23. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries

    Master-uppsats, Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Nicole Eger; [2021]
    Nyckelord :Genotyping; Genomic barcodes; LAMP; PNA; RCA; FISH;

    Sammanfattning : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. LÄS MER

  5. 24. Hypoxia-induced expression of HIF-1a and GPR30, in relation to survival, proliferation and apoptosis in MCF-7 and SkBr3 breast cancer cells.

    L3-uppsats, Lunds universitet/Läkarutbildningen

    Författare :Hanna Chahin; Dianna Jazera; [2021]
    Nyckelord :hypoxia; breast cancer; GPR30 estrogen receptor; Medicine and Health Sciences;

    Sammanfattning : Introduction: Hypoxia that accompanies tumor growth induces the expression of hypoxiainducible factor a (HIF-1a), in turn mediating the expression of other genes, including G protein-coupled receptor 30 (GPR30), to promote adaption to the hypoxic microenvironment and enable cell proliferation and survival. Nonetheless, this correlation has not been causally explained. LÄS MER

  7. 25. UTVÄRDERING AV FLUORESCERANDE PROTEINER I DET RÖDA SPEKTRUMET SOM MARKÖRER I GENETISKA KRETSAR SKAPADE FÖR IMMUNTERAPI AV CANCER

    Kandidat-uppsats, Malmö universitet/Fakulteten för hälsa och samhälle (HS)

    Författare :Caroline Persson; [2021]
    Nyckelord :BFP; CAR T-celler; Fluorescerande protein; genetisk krets; GFP; immunterapi; plasmid; Lentivirus;

    Sammanfattning : Fluorescerande protein kan användas som reportrar i genetiska kretsar för att identifiera framgångsrikt modifierade celler. Med hjälp av syntetisk biologi kan genetiska kretsar, som är en sammansättning gener som kodar för protein, skapas. LÄS MER