Sökning: "Protein Purification"

Visar resultat 1 - 5 av 121 uppsatser innehållade orden Protein Purification.

  1. 1. The production of recombinant 6LZE_A and NS2B-NS3 in E. coli

    Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Författare :Raluca Svensson; [2024]
    Nyckelord :coronavirus; dengue; protease; pandemic; SARS-Cov-2; DENV; viral replication; proteins; Escherichia coli; DL-BAPNA; computational modeling; recombinant protein production; biotechnology; Biology and Life Sciences;

    Sammanfattning : Even though the first coronavirus pandemic was registered in 1965, SARS-CoV-2 was better acknowledged when it caused the worldwide pandemic in 2019 in which millions of individuals lost their lives. Due to its facile way of spreading from one individual to another, the number of infected cases has gone up to more than 750 million to this day. LÄS MER

  3. 2. Effects of phosphorylation on the ADP-ribosylation activity of PARP16

    Kandidat-uppsats, Lunds universitet/Kemiska institutionen

    Författare :Virginia Martín Cristóbal; [2023]
    Nyckelord :ADP-ribosylation; Kinases; PARP16; Phosphorylation; UPR biochemistry; Biochemistry; Chemistry;

    Sammanfattning : PARP16/ARTD15 is an intracellular mono-ADP-ribosyltransferase that catalyzes auto- and heteromodification by transfer of a single ADP-ribose moiety. It belongs to the ADP-ribosyltransferase (ART) superfamily. LÄS MER

  4. 3. Characterization of Antigenic Properties of Two Immunogenic Proteins of Streptococcus pneumoniae 

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Mawj Jasimalsalih; [2023]
    Nyckelord :Immunogenic Proteins; PrsA; MalX; Membrane Vesicles; Protein Properties; Streptococcus pneumoniae virulence factors;

    Sammanfattning : The bacterium Streptococcus pneumoniae (pneumococcus), is considered to be a leading cause of morbidity and mortality globally, particularly in infants and the elderly. It is one of the most frequent causes of respiratory tract infections, which sporadically have the potential to develop into serious invasive symptoms including sepsis and meningitis. LÄS MER

  5. 4. Development of specific host cell protein assays

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Ivert Nordén; [2023]
    Nyckelord :Gyrolab; Immunoassay; HCP;

    Sammanfattning : The manufacturing and the development of biotherapeutic drugs involves the expression of biotherapeutic proteins in a host cell expression system followed by a purification process. Bioanalytical methods to measure impurities such as host cell proteins (HCPs) are needed to obtain a robust process and a safe drug according to regulatory requirements. LÄS MER

  7. 5. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER