Sökning: "Protien purification"

Hittade 1 uppsats innehållade orden Protien purification.

  1. 1. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Erik Apelqvist; [2022]
    Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER