Sökning: "RNA-seq"

Visar resultat 1 - 5 av 15 uppsatser innehållade ordet RNA-seq.

  1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Alexander Westlund; [2019]
    Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER

  2. 2. Gene expression patterns in Fraxinus excelsior clones with contrasting susceptibility to Hymenoscyphus fraxineus

    Master-uppsats, SLU/Dept. of Forest Mycology and Plant Pathology

    Författare :Shadi Eshghi Sahraei; [2016]
    Nyckelord :Hymenoscyphus fraxinus; Fraxinus excelsior; ABA8H; RNA-seq; ATAF1;

    Sammanfattning : This study aimed at investigating gene expression patterns in resistant and susceptible clones of Fraxinus excelsior L. (European ash) in response to infection with Hymenoscyphus fraxineus. A further objective of this study was to investigate the role of ABA8-hydroxylase genes (ABA8H) in European ash under infection with H. fraxineus. LÄS MER

  3. 3. Integrative analysis of age-related changes in the transcriptome of Caenorhabditis elegans

    Magister-uppsats, Högskolan i Skövde/Institutionen för biovetenskapHögskolan i Skövde/Forskningscentrum för Systembiologi

    Författare :Tsimafei Padvitski; [2015]
    Nyckelord :Aging; gene expression; RNA-seq; transcription regulation;

    Sammanfattning : Ageing is difficult to study because of the complexity and multi-factorial nature of traits that result from a combination of environmental, genetic, epigenetic and stochastic factors, each contributing to the overall phenotype. In light of this challenge, transcriptomic studies of aging organisms are of particular interest, since transcription is an intermediate step that links genotype and phenotype. LÄS MER

  4. 4. Gene expression in European Ash in response to inoculation with Hymenoscyphus pseudoalbidus

    Magister-uppsats, SLU/Dept. of Forest Mycology and Plant Pathology

    Författare :Shadi Eshghi Sahraei; [2015]
    Nyckelord :European ash; Hymenoscuphus pseudoalbidus; RNA-sequences; RNA-sequences data;

    Sammanfattning : European Ash is one of the economically important tree in Europe which is threatened by an invasive pathogen namely Hymenoscyphus pseudoalbidus, causing Ash dieback disease. Our objective was to investigate genes that are expressed after inoculation with this fungus and with freezing injury. LÄS MER

  5. 5. RNA-Seq: Yellow Nutsedge (Cyperus esculentus) transcriptome analysis of lipid-accumulating tubers from early to late developmental stages

    Master-uppsats, SLU/Dept. of Animal Breeding and Genetics

    Författare :Axel Thieffry; [2014]
    Nyckelord :Cyperus esculentus; transcriptome; tubers; RNA-seq; bioinformatics;

    Sammanfattning : Thanks to high amounts of starch and oil amassed in the parenchyma of its tubers, yellow nutsedge (Cyperus esculentus) stands as a unique plant species with regards to nutrient biosynthesis and accumulation in underground organs. In the last decades, understanding of enzymatic processes in lipid, starch and sugar pathways underwent great improvements. LÄS MER