Sökning: "RT qPCR"

Visar resultat 11 - 15 av 60 uppsatser innehållade orden RT qPCR.

  1. 11. Inducering av interferon-gamma och tumörnekrosfaktor-alfa i helsaliv : En icke-invasiv metod för att diagnostisera celiaki

    Kandidat-uppsats, Malmö universitet/Institutionen för biomedicinsk vetenskap (BMV)

    Författare :Dorina Kokrehel; [2022]
    Nyckelord :celiac disease; interferon-gamma; saliva; tumor necrosis factor alpha; celiaki; gliadin; IFN-γ; saliv; TNF-⍺;

    Sammanfattning : Celiaki (glutenintolerans) är en kronisk, autoimmun sjukdom med diffusa symtom. Vid förtäring av glutenhaltig mat uppstår en allergisk reaktion hos glutenintoleranta individer. Gluten kan inte fullständigt brytas ned av kroppens enzymer, vilket betyder att icke nedbrutna peptidfragment (såsom glutamin) absorberas i tarmslemhinnan. LÄS MER

  3. 12. Evaluation of the Two-tailed RT-qPCR method with the application of manual vs robotic extraction of miRNA

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Lia Rozenberg; [2022]
    Nyckelord :;

    Sammanfattning : Sepsis is aggressive and severe inflammatory body response to an infection and is considered to be one of the most common death causes in patients. The current diagnosis of sepsis is not fast enough to help those who get sepsis, due to its fast progression. The current golden standard for sepsis diagnosis is blood culturing. LÄS MER

  4. 13. Recombinant Expression of Novel Wild Type Oat Hemoglobins in Escherichia Coli

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Frank Rummukainen; [2022]
    Nyckelord :Recombinant technology; Protein expression; Hemoglobin; Phytoglobin; Escherichia coli; Oat; Avena sativa; Heme; Iron; Biosynthesis; Transcription; qPCR; SDS-PAGE; Fermentation; Recombination; Applied Biochemistry; Biology and Life Sciences;

    Sammanfattning : Hemoglobin is a protein transporting oxygen through our bloodstreams and is as such essential for aerobic life. Hemoglobins also exist in plants, with a wide variety of functions other than oxygen transport. LÄS MER

  5. 14. Future diagnostic of Sepsis : Biomarker miRSeps-5 manual vs robotic extraction and optimization of miRNA detection by Two-Tailed RT-qPCR technology

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Erika Kasaras; [2022]
    Nyckelord :;

    Sammanfattning : Sepsis is a syndrome developed in response to infection when a body’s immune system cannot adequately respond to infection. To this day, sepsis holds a peak position in mortality rates worldwide. Currently, SOFA score and blood culturing as means to diagnose sepsis are standard. LÄS MER

  7. 15. Manual and robotic RNA extraction from human plasma with absolute quantification of miRNA through two-tailed RT-qPCR as part of research into early diagnosis of sepsis

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Lourens Groenewald; [2022]
    Nyckelord :;

    Sammanfattning : Each hour´s delay in administering antibiotics has been shown to result in a 9% increase in the odds of mortality in sepsis cases. It is thus evident that the development of a diagnostic method that ensures an early time to diagnosis of sepsis is essential. LÄS MER