Sökning: "Recombinant technology"

Visar resultat 1 - 5 av 48 uppsatser innehållade orden Recombinant technology.

  1. 1. Recombinant Expression of Novel Wild Type Oat Hemoglobins in Escherichia Coli

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Frank Rummukainen; [2022]
    Nyckelord :Recombinant technology; Protein expression; Hemoglobin; Phytoglobin; Escherichia coli; Oat; Avena sativa; Heme; Iron; Biosynthesis; Transcription; qPCR; SDS-PAGE; Fermentation; Recombination; Applied Biochemistry; Biology and Life Sciences;

    Sammanfattning : Hemoglobin is a protein transporting oxygen through our bloodstreams and is as such essential for aerobic life. Hemoglobins also exist in plants, with a wide variety of functions other than oxygen transport. LÄS MER

  3. 2. Evaluation of strategies for construction of protease libraries in yeast.

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Författare :Sidharth Jaya Sankar; [2022]
    Nyckelord :Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Sammanfattning : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. LÄS MER

  4. 3. Flödescytometrisk undersökning av inbindning mellan designade topdomänen från transferrinreceptorn till virala glykoproteiner för potentiell användning inom läkemedelsframtagning

    Kandidat-uppsats, Linnéuniversitetet/Institutionen för kemi och biomedicin (KOB)

    Författare :Emma Rydell; [2022]
    Nyckelord :Transferrin receptor; recombinant DNA techniques; protein purification; yeast surface display; flow cytometry; Transferrinreceptor; rekombinant genteknik; proteinupprening; yeast surface display; flödescytometri;

    Sammanfattning : Machupovirus är ett virus som kan orsaka hemorragisk feber hos människor. Efter utvärdering av bindning mellan designade proteiner och virala glykoproteiner skulle proteinerna potentiellt kunna användas vid framtagning av ett proteinbasserat läkemedel mot hemorragisk feber. LÄS MER

  5. 4. Selection and Characterisation of Affibody Molecules Intended for Drug Conjugates Targeting Cancer Cells

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Elin Hedberg; [2022]
    Nyckelord :Affibody; AffiDC; affibody-drug conjugate; urothelial cancer; cell display; affibody library;

    Sammanfattning : Affibodymolekyler är små affinitetsproteiner (6.5 kDa) som föreslås kunna ersätta monoklonala antikroppar i terapeutiska tillämpningsområden, exempelvis i antikropp-läkemedelskonjugat (ADCs) som kan navigera sig fram till biomarkörer som är uttryckta på cancerceller. LÄS MER

  7. 5. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

    Master-uppsats, Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Författare :Aakash Reddy Murugadasan; [2022]
    Nyckelord :Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

    Sammanfattning : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. LÄS MER