Sökning: "Rolling Circle Amplification"
Visar resultat 1 - 5 av 19 uppsatser innehållade orden Rolling Circle Amplification.
1. A novel assay for the detection of Hepatitis C virus in blood plasma, using padlock probes and rolling circle amplification
Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskapSammanfattning : Hepatitis C viral infection is a globally widespread blood-borne disease affecting the liver, causing cirrhosis and hepatocellular carcinoma. This type of liver cancer is mainly caused by chronic Hepatitis C and Hepatitis B. Specific detection with the following correct treatment is crucial to reduce the overall burden of the disease. LÄS MER
2. Quantification of DNA Nanoballs Using Image Processing Techniques
Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Avdelningen Vi3Sammanfattning : In gene editing, it is important to identify the number of edited and unedited nucleic acids in the development of new therapies and drugs. Countagen is developing a technology for accelerating genomic research and their product is called GeneAbacus. LÄS MER
3. Quantification of DNA Microballs Using Image Processing Techniques
Master-uppsats, KTH/Industriell bioteknologiSammanfattning : I detta examensarbete användes olika bildbehandlingstekniker för detektion och kvantifiering av DNA-mikrobollar, mer specifikt rolling circle amplification-produkter, på mikroskopibilder. Avsikten med detta arbete var att hjälpa Countagen AB utforska pipelines för bildbehandling för sin produkt där de analyserar utfallet av genredigeringsförsök på ett billigare och snabbare sätt än dagens konventionella sekvenseringsmetoder. LÄS MER
4. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries
Master-uppsats, Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildningSammanfattning : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. LÄS MER
5. Circle-to-circle amplification to improve the sensitivity of a magnetic nanoparticle-based DNA detection protocol
Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/MolekylärbiologiSammanfattning : Magnetic nanoparticles have great potential in the biomedical and diagnostics field. Due to their small size, the particles have a high surface-to-volume ratio which enables for biofunctionalisation with different molecular probes. This makes itpossible to target them against a wide variety of biomarkers. LÄS MER