Sökning: "Samuel Sutiono"

Hittade 1 uppsats innehållade orden Samuel Sutiono.

  1. 1. Enzyme engineering of a haloacid dehalogenase-like phosphatase from Thermotoga neopolitana for optimization of substrate specificity

    Master-uppsats, Lunds universitet/Bioteknik (master)

    Författare :Samuel Sutiono; [2016]
    Nyckelord :Catalytic efficiency; Haloacid dehalogenase-like hydrolase; Enzyme engineering; Thermal stability; Inhibition; biotechnology; bioteknik; Technology and Engineering;

    Sammanfattning : Haloacid dehalogenase (HAD)-like hydrolases represent a vast superfamily characterised by their ability to form covalent enzyme-substrate intermediates facilitating the cleavage of C-Cl, C-P, or CO-P bonds. Within this work one member of this superfamily, HAD from Thermotoga neopolitana (HADTn) exhibiting a phosphatase (P-O bond cleavage) like activity was investigated in the context of altering the substrate specificity. LÄS MER