Sökning: "Shahin Ghoncheh"

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  1. 1. Measurement of ethanol in microdialysis samples by means of enzymatic assay using alcohol dehydrogenase and NAD

    Magister-uppsats, Högskolan i Borås/Institutionen Ingenjörshögskolan

    Författare :Shahin Ghoncheh; [2008]
    Nyckelord :alcohol dehydrogenase; ethanol; four-parameter logistic model; microdialysis; blood flow changes;

    Sammanfattning : The enzymatic method for ethanol measurement can detect very low concentration of ethanol at samples, consequently it can’t be applied for samples with high concentration and implies as very sensitive method at limited range of detection.The alcohol dehydrogenase method is based on oxidation of alcohol in the presence of ADH as enzyme and NAD+ as coenzyme and formation of acetaldehyde and NADH that can be monitored by spectrophotometric measurement at 334,340 or 365 nm wavelengths. LÄS MER