Sökning: "Single-molecule fluorescence microscopy"

Visar resultat 1 - 5 av 6 uppsatser innehållade orden Single-molecule fluorescence microscopy.

  1. 1. Study of Automated Step Detection Methods and Dwell Time Analysis of Single-Molecule Data : Case of Study: The Turnover of Single Fluorescent ATP on The Active Site of Myosin

    Master-uppsats, Linnéuniversitetet/Institutionen för fysik och elektroteknik (IFE)

    Författare :Lathifa Nur Ramdhania; [2022]
    Nyckelord :Single-molecule; step detection; dwell time analysis;

    Sammanfattning : Single-molecule methods were developed with the objective to obtain kinetic information of biomolecular processes from a single biomolecule. This objective can be achieved by analyzing the binding events in recordings (“trajectories”) of single molecules (e.g., using fluorescence); the so-called dwell times. LÄS MER

  3. 2. Improving the temporal resolution of a microspectrometer for the study of the photophysics of enhanced green fluorescent protein

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Lukas Rane; [2021]
    Nyckelord :Biophysics; EGFP; Fluorescence; triplet state; Biofysik; EGFP; fluorescens; triplet tillstånd;

    Sammanfattning : The use of fluorescent proteins as fluorescent markers has exploded over the last decades. In particular due to the development of advanced microscopy for live cell measurements, dynamic molecular studies down to single molecule levels and for superresolution microscopy. LÄS MER

  4. 3. Development of a single-molecule tracking assay for the lac repressor in Escherichia coli

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för cell- och molekylärbiologi

    Författare :Oscar Broström; [2019]
    Nyckelord :Escherichia Coli; Single-molecule fluorescence microscopy; In vivo tracking; In vivo; lac repressor; LacI; Bifunctional rhodamine B; E. coli; In vivo;

    Sammanfattning : Gene regulation by transcription factors are one of the key processes that are important to sustain all kinds of life. In the prokaryote Escherichia coli this has shown to especially crucial. The operator sequence to which these transcription factors bind to are very small in comparison to the whole genome of E. LÄS MER

  5. 4. Construction of a Scanning Laser Confocal Flourescence Microscope System for Single Molecule Detection and Spectroscopy

    Master-uppsats, Lunds universitet/Avdelningen för Biomedicinsk teknik

    Författare :Erik Hedlund; [2011]
    Nyckelord :Technology and Engineering;

    Sammanfattning : The Single Molecule Spectroscopy Group at the department of Chemical Physics in the Chemical Centre of Lund University conducts research involving spectroscopy on single molecules of organic dyes. Fluorescence microscopy using a wide-field scheme has been used and such a scheme has many advantages such as full image capture at once when using a high sensitivity CCD or CMOS camera. LÄS MER

  7. 5. Fluorescence Lifetime Measurement using Time Correlated Single Photon Counting

    Master-uppsats, Lunds universitet/Atomfysik; Lunds universitet/Kemisk fysik

    Författare :Mohammad Bilal Akhtar; [2010]
    Nyckelord :Chemistry;

    Sammanfattning : A time correlated single photon counting (TCSPC) setup is built to measure the fluorescence decay of samples, such as solar cell materials. TCSPC is a sensitive technique for measuring fluorescence decays on nanosecond time scale and longer. LÄS MER