Sökning: "TIRF microscopy"

Hittade 3 uppsatser innehållade orden TIRF microscopy.

  1. 1. Study of Automated Step Detection Methods and Dwell Time Analysis of Single-Molecule Data : Case of Study: The Turnover of Single Fluorescent ATP on The Active Site of Myosin

    Master-uppsats, Linnéuniversitetet/Institutionen för fysik och elektroteknik (IFE)

    Författare :Lathifa Nur Ramdhania; [2022]
    Nyckelord :Single-molecule; step detection; dwell time analysis;

    Sammanfattning : Single-molecule methods were developed with the objective to obtain kinetic information of biomolecular processes from a single biomolecule. This objective can be achieved by analyzing the binding events in recordings (“trajectories”) of single molecules (e.g., using fluorescence); the so-called dwell times. LÄS MER

  3. 2. Mapping the relationship between integrin activation, focal adhesion organization and mechanotransduction in fibroblasts

    Master-uppsats, Lunds universitet/Avdelningen för Biomedicinsk teknik

    Författare :Valeriia Grudtsyna; [2021]
    Nyckelord :Mechanotransduction; Focal adhesions; Yes-associated protein 1 YAP ; Integrins; Fibronectin; Polarization microscopy; TIRF microscopy; Fluorescence anisotropy; YAP activation; Image analysis; Cell biology; Technology and Engineering;

    Sammanfattning : Cells can sense and respond to mechanical forces through a process called mechanotransduction. It is controlled by the mechanical cues, originated from the cell's microenvironment, and occurs via a complex, step-wise process. LÄS MER

  4. 3. Lipid Bilayers on Planes and in Micropipettes - Two model systems to study binding of DivIVA to flat and negatively curved membranes

    Master-uppsats, Lunds universitet/Fysikalisk kemi; Lunds universitet/Fysiska institutionen

    Författare :Elisabeth Baumann; [2016]
    Nyckelord :supported lipid bilayer; DivIVA; curvature; TIRF; FRAP; epifluorescence microscopy; micropipette; model system; Physics and Astronomy;

    Sammanfattning : The aim of this thesis work was to form and characterize model systems of cell membranes on planar supports and in micropipettes. Firstly, supported lipid bilayers (SLBs) were formed on glass slides after an existing experimental procedure. LÄS MER