Sökning: "Taqman"

Visar resultat 1 - 5 av 21 uppsatser innehållade ordet Taqman.

  1. 1. Development of a novel quantitative PCR analysis method for HIV-1

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Anna-Malin Hallberg; [2021]
    Nyckelord :HIV; PCR; primers; probes; virus; detection; blood plasma; pathogen; biological pharmaceutical; protein-based pharmaceutical; genotype; subtype; RT qPCR; extraction; TaqMa; RNA virus; cross-dimer; applied biochemistry; tillämpad biokemi; Biology and Life Sciences; Technology and Engineering; Chemistry;

    Sammanfattning : Biological pharmaceuticals are the answer to many severe diseases of today’s society. Some of these are protein-based drugs and can be produced from human blood plasma. In order to guarantee the safety of patients, absence of any pathogens needs to be ensured. LÄS MER

  2. 2. Development and validation of a novel quantitative PCR analysis method for HIV-1

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Josefine Sundgren; [2020]
    Nyckelord :HIV-1; qPCR; Octapharma; Taqman; applied biochemistry; tillämpad biokemi; Technology and Engineering;

    Sammanfattning : The major problem when developing primers and probe sets for detection of HIV-1 is its high genetic variability and strong ability to mutate. HIV-1 is divided into four groups, M, N, O, P based on genetic similarities, and there are more than 70 different circulating recombinant forms. LÄS MER

  3. 3. Optimisation and Validation of PCR Method for HLA Gene Expression to Enable PCR System Transfer and Master Mix Change

    Kandidat-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Paulina Odlander; [2020]
    Nyckelord :HLA; celiac disease; DQ-genes; PCR; PCR optimisation;

    Sammanfattning : Health Tech company Dynamic Code AB provides a PCR test for determination of HLA DQ-genes connected to development of celiac disease. The PCR method is probe based and in real time and is at this time carried through on the, somewhat outdated, PCR instrument from Thermo Fisher/Applied Biosystems called 7300 Real-Time PCR System. LÄS MER

  4. 4. The Effect of Probiotic Bacteria on High Sugar Diet Induced Diabetes Type-2 Symptoms in Fruit Fly : With Focus on Lipid Metabolism

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för hälsa och lärande

    Författare :Rasiat Umar Sambo; [2019]
    Nyckelord :Probiotic Bacteria; Diabetes type 2; High Sugar Diet; Drosophila melanogaster;

    Sammanfattning : As the prevalence of type 2 diabetes has increased globally, so has the need to further investigate the disorder’s underlying features and a potential target for treatment. Drosophila melanogaster has proven to be an excellent model organism to study type 2 diabetes (T2D). LÄS MER

  5. 5. Is a potential regulatory region in the vicinity of EDN3 linked to the performance in Standardbred horses?

    Master-uppsats, SLU/Dept. of Animal Breeding and Genetics

    Författare :Linda Novácková; [2018]
    Nyckelord :performance; standardbred; EDN3; endothelin-3;

    Sammanfattning : Standardbred horses (SBs) are used in harness racing. Their common breeding history with Coldblooded trotters (CBTs) led us to the hypothesis, that the same region associated with race performance in CBTs, may be linked to the performance of SBs as well. EDN3 SNP (g.22:45748491C>T), was found to be associated (p. LÄS MER