Sökning: "affinitetskromatografi"

Visar resultat 1 - 5 av 11 uppsatser innehållade ordet affinitetskromatografi.

  1. 1. Scale-down modelling of the upstream process for production of Affibody® Molecules

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Philip Masreliez; [2022]
    Nyckelord :Scale-down modelling; protein expression; Affibody® molecule; FeedPlate®; EnPresso® B; STR; Nedskalad produktionsmodell; proteinuttryck; Affibodymolekyler; FeedPlate®; EnPresso® B; STR;

    Sammanfattning : I detta projekt har uttrycket av Affibodymolekyler i bioreaktorer av olika volymetriska skalor jämförts för att fastställa om ett tillförlitligt samband mellan de olika bioreaktorernas prestanda kan etableras för att möjliggöra utvecklingen av en nedskalad produktionsmodell för Affibodymolekyler. Baslinjen för jämförelsen i denna studie har varit en enliters- stirred tank reactor (STR) som de andra (mindre) bioreaktorernas prestanda jämfördes med. LÄS MER

  3. 2. Simulation of periodic counter-current chromatography purification of monoclonal antibodies with column degradation

    Master-uppsats, Lunds universitet/Kemiteknik (CI)

    Författare :Erik Friberg; [2022]
    Nyckelord :Counter-current chromatography; PCC; Monoclonal antibodies; Affinity chromatography; Process control; Chemical engineering; Process simulation; Chemistry;

    Sammanfattning : The purification process of monoclonal antibodies (mAbs) for therapeutic drugs is expensive, especially the chromatography step in which the mAbs are separated from other proteins and cells present in the feed. The antibodies are separated from the feed using affinity chromatography, where only the antibodies interact with active sites within the column, whereas the rest passes through the column without stopping. LÄS MER

  4. 3. Small Scale Monoclonal Antibody Purification Platform

    Master-uppsats, Lunds universitet/Kemiteknik (CI)

    Författare :Eric Nielsen; [2022]
    Nyckelord :PURIFICATION; MONOCLONAL ANTIBODIES; ÄKTA PURE; ÄKTA XPRESS; Chemistry;

    Sammanfattning : The aim of this project is to transfer and implement a monoclonal antibody purification process that is traditionally done on an ÄKTAxpress to another machine called the ÄKTA pure. This is due to the fact that the ÄKTAxpress is being discontinued by the manufacturer and a replace-ment machine is needed for the time being. LÄS MER

  5. 4. Investigation of possible improvements of the stability of bispecific ADAPT proteins

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Ella Eriksson; [2021]
    Nyckelord :Protein purification; Affinity chromatography; ABD-derived domain; ADAPT protein; simultan binding;

    Sammanfattning : The aim of the project was to identify positions and amino acids that contribute to improved structure and stability of bispecific ADAPT proteins. During the 20 weeks project period, different amino acid substitutions were analysed to evaluate the effect on the three-helical structure and stability of bispecific ADAPTs targeting human serum albumin (HSA) and tumor necrosis factor α (TNFα). LÄS MER

  7. 5. Comparison of Lectins and their suitability in Lectin Affinity Chromatography for isolation of Glycoproteins

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Pontus Andersson; [2020]
    Nyckelord :biotechnology; lectins; chromatography; glycoproteins; lectin affinity chromatography; LAC; ConA; Concanavalin A; bioteknik; lektiner; kromatografi; affinitetskromatografi; glykoproteiner; ConA; Concanavalin A;

    Sammanfattning : Virtually all extracellular proteins in humans are glycoproteins and likewise are many biopharmaceuticals. The glycosylation is directly correlated to biological function and stability of these proteins. The ability to isolate glycoproteins is thus of great importance in many applications. LÄS MER