Sökning: "affinity determination"

Visar resultat 1 - 5 av 15 uppsatser innehållade orden affinity determination.

  1. 1. Optimization of two high-performance size-exclusion chromatography methods with diode-array detection for protein quantification and purity assessment

    Master-uppsats, Lunds universitet/Centrum för analys och syntes

    Författare :Camilla Lane; [2023]
    Nyckelord :Method qualification; Protein concentration; Protein Purity; High Performance Size exclusion chromatography; Quality control; Analytical chemistry; Biology and Life Sciences;

    Sammanfattning : Background: There is a need to develop methods of determining concentration and purity for two new affinity ligands, protein T and protein H, in order to ensure the quality of an industrial production of said proteins. Aims: To develop a High Performance Size Exclusion Chromatography, HPSEC, method to determine concentration and purity of protein T and to improve an existing HPSEC method for concentration and purity determination of protein H that show selectivity, display linearity between peak area and injection amount and give repeatable results. LÄS MER

  3. 2. Trivsel och motivation. En kvalitativ studie om vad som påverkar anställdas trivsel och motivation på arbetet

    Kandidat-uppsats, Göteborgs universitet/Företagsekonomiska institutionen

    Författare :Walid Samadi; Carl Hallgren; [2022-08-16]
    Nyckelord :Job satisfaction; job motivation and leadership;

    Sammanfattning : The art of motivating and engaging a group of people has been an important function throughout human history. In recent decades, skilled labor has become more and more important for companies and organizations around the world. The purpose of this study is to find out what factors are vital for employees to feel motivated and love their jobs. LÄS MER

  4. 3. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Erik Apelqvist; [2022]
    Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

  5. 4. Lågpresterande elevers syn på den egna prestationen i idrott och hälsa : En kvalitativ studie om motivation till ämnet idrott och hälsa

    Magister-uppsats, Gymnastik- och idrottshögskolan, GIH/Institutionen för rörelse, kultur och samhälle

    Författare :Jasmin Burrau; [2022]
    Nyckelord :motivation; idrott och hälsa; motivationsfaktorer; lågpresterande elever;

    Sammanfattning : Aim The purpose of the study is to investigate and describe how low-performing students in year 9 look at their achievements in connection with teaching in sport and health. Furthermore, it is of interest to examine which motivational factors influence them to participate or not participate in the teaching. LÄS MER

  7. 5. Reducerad aggregering av affibodymolekyler

    Kandidat-uppsats, KTH/Medicinteknik och hälsosystem

    Författare :Josefin Rehn Hamrin; Nina Gyllenhoff; [2022]
    Nyckelord :Affibody; solubility; precipitation; aggregation prone; concentration determination; Affibody; löslighet; aggregerande protein; koncentrationsbestämning;

    Sammanfattning : I dagsläget utvecklas ständigt nya läkemedel med hjälp av affinitetsprotein. På 90-talet skapades ett nytt sådant protein utvunnet från Protein A på KTH, den så kallade affibodyn. Att arbeta med affibodies är ofta fördelaktigt eftersom de är lätta producera samtidigt som de delar många av de väsentliga egenskaperna hos en antikropp. LÄS MER