Sökning: "cation exchange chromatography."

Visar resultat 1 - 5 av 9 uppsatser innehållade orden cation exchange chromatography..

1. 1. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

   Författare :Diyar Ayranci; [2021]
   Nyckelord :Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Sammanfattning : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. LÄS MER

3. 2. Identification and Expression of Oat Hemoglobin

   Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

   Författare :Simon Christensen; [2019]
   Nyckelord :Hemoglobin; Oat; Plant Hemoglobin; Hemoglobin-based oxygen carrier; non-symbiotic Hemoglobin; oxygen transporter; applied biochemistry; Biology and Life Sciences; Technology and Engineering; Medicine and Health Sciences;

   Sammanfattning : Hemoglobin is mainly viewed as an oxygen and carbon dioxide carrier in the blood of animals and thus essential for life as we know it. During the course of evolution, plants have also developed versions of this protein, for similar and different purposes, such as regulation of nitric oxide levels. LÄS MER

4. 3. Characterisation of free and conjugated protease inhibitors from Solanum tuberosum

   Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för kemi - BMC

   Författare :Kristoffer Lundmark; [2017]
   Nyckelord :enzyme; trypsin; chymotrypsin; pancrease protease; serine protease inhibitors; SPI; potato tubers; potato; Solanum tuberosum; immobilized; aluminium oxide membranes; inhibitor-enzyme interaction;

   Sammanfattning : The main purpose of the master thesis project is to investigate the influence of selected serine protease inhibitors (SPI) on the catalytic action of the serine proteases chymotrypsin and trypsin, in a conjugated and non-conjugated state. The inhibitors included for this study were extracted from Solanum tuberosum, i.e.common potato. LÄS MER

5. 4. Cloning, expression and production of Vicia faba Leghemoglobin B

   Master-uppsats, Lunds universitet/Tillämpad biokemi

   Författare :Gustav Lindéus; [2016]
   Nyckelord :blood substitute; fermentation; gene technology; Leghemoglobin; hemoglobin; Vicia faba; applied biochemistry; tillämpad biokemi; Biology and Life Sciences;

   Sammanfattning : In this thesis a preliminary evaluation of the potential of a leghemoglobin (Lb) for the development of a blood substitute in humans, resembling hemoglobin (Hb) in red blood cells, was conducted. Leghemoglobin B (VfLbB), a gene from Vicia faba, was cloned into a pET-DEST42 vector, using the Gateway™ recombination technology. LÄS MER

7. 5. Investigation and optimization of elution buffer effects on the elution profile for the cation exchange chromatography resin POROS XS : In association with Cobra Biologics

   Master-uppsats, KTH/Skolan för bioteknologi (BIO)

   Författare :Heidi Lemström; [2016]
   Nyckelord :;

   Sammanfattning : The goal of this project was to investigate how elution buffer properties affectthe protein elution in the polishing purification step with a cation exchange chromatographyconsisting of POROS XS resin. Hence be able to better predict thebehavior of the resin, two model proteins were used for this investigation (K100and Y102). LÄS MER