Sökning: "cell biology"

Visar resultat 1 - 5 av 154 uppsatser innehållade orden cell biology.

  1. 1. Sex-specific pathological response to Traumatic Brain Injury

    Master-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Christopher Fry; [2022]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Traumatic brain injury (TBI) is one of leading causes of death in the world; survivors commonly exhibit behavioural changes and neurocognitive defects in the long-term. Following TBI, rapid changes in the neurovascular unit (NVU) occur as the permeability of the blood brain barrier (BBB) increases and cerebral blood flow (CBF) becomes dysregulated. LÄS MER

  2. 2. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Författare :Erik Apelqvist; [2022]
    Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

  3. 3. Developing and validating assay-ready HEK-Blue CD40L cells : For a more flexible and faster screening of Affibody® molecules

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Författare :Erika Könberg; [2022]
    Nyckelord :cell biology; assay-ready cells; HEK-Blue cells;

    Sammanfattning : Cell assay that evaluates a biologics’ potency and efficacy is an important part in the discovery and development of drug candidates. However, it requires regular maintenance of cell cultures, and the cell assays can only be performed when the cells have reached 70-80% confluency. LÄS MER

  4. 4. Recombinant Expression of Novel Wild Type Oat Hemoglobins in Escherichia Coli

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Teoretisk kemi

    Författare :Frank Rummukainen; [2022]
    Nyckelord :Recombinant technology; Protein expression; Hemoglobin; Phytoglobin; Escherichia coli; Oat; Avena sativa; Heme; Iron; Biosynthesis; Transcription; qPCR; SDS-PAGE; Fermentation; Recombination; Applied Biochemistry; Biology and Life Sciences;

    Sammanfattning : Hemoglobin is a protein transporting oxygen through our bloodstreams and is as such essential for aerobic life. Hemoglobins also exist in plants, with a wide variety of functions other than oxygen transport. LÄS MER

  5. 5. Classification of sequence tags from tandem mass spectrometry spectra using machine learning models

    Master-uppsats, Lunds universitet/Examensarbeten i bioinformatik

    Författare :Júlia Ortís Sunyer; [2022]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Motivation: Proteomics is the large-scale study of all the proteins found in a cell, tissue or organism. In the last few years, and thanks to the development of mass spectrometry and bioinformatics, proteomics has led the research in several fields, ranging from medicine to agriculture. LÄS MER