Sökning: "cryo-em"

Visar resultat 1 - 5 av 15 uppsatser innehållade ordet cryo-em.

  1. 1. Characterization of Mitilysin Pores by Cryo-electron Microscopy

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Vladimir Novakovic; [2023]
    Nyckelord :Mitilysin; Mly; Structural Biology; Cryo-EM; pore; toxin; pore forming proteins; pore forming toxins; PFP; PFT;

    Sammanfattning : Pore forming toxins (PFTs) are a large group of proteins found mainly in bacteria with some exceptions found in animals. They bind and form pores in their target membranes and form pores, which leads to cell death. Among these are cholesterol-dependent cytolysins (CDC), which require the presence of cholesterol to bind target membranes. LÄS MER

  3. 2. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    Master-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Anna Králová; [2023]
    Nyckelord :time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Sammanfattning : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. LÄS MER

  4. 3. Approaches to Structural Characterization of a Heteromeric GABA(A)R

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Alexander Stevens; [2023]
    Nyckelord :Applied Physics; Biophysics; Molecular Biophysics; GABA A R; LGIC; pLGIC; Tillämpad Fysik; Biofysik; Molekylär Biofysik; GABA A R; LGIC; pLGIC;

    Sammanfattning : Structural biology has become an important part of researching various diseases and drug development. In this thesis, I provide details on how I worked with approaches to structural characterization of a heteromeric GABA(A)R. LÄS MER

  5. 4. Expression and purification of pyruvate transporting human mitochondrial pyruvate carrier (MPC) protein complex for structural studies

    Magister-uppsats, Lunds universitet/Examensarbeten i molekylärbiologi

    Författare :Markus Wendler; [2022]
    Nyckelord :Biology and Life Sciences;

    Sammanfattning : Pyruvate is one of the main molecules needed for the citric acid cycle, which produces energy in the form of ATP. The citric acid cycle reaction occurs in the mitochondrial matrix, and for pyruvate to participate in the reaction, it needs to be transported from the cytosol across the mitochondrial membranes into the mitochondrial matrix. LÄS MER

  7. 5. Engineered imaging scaffolds for cryo-EM of small proteins of interest

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Oscar Friberg; [2022]
    Nyckelord :Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

    Sammanfattning : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. LÄS MER