Sökning: "e coli transformation"

Visar resultat 1 - 5 av 11 uppsatser innehållade orden e coli transformation.

1. 1. Cloning and expressing the genes encoding Glycerol dehydratase (GDHt) and 1,3-Propanediol dehydrogenase (1,3-PDDH) in E. coli

   Master-uppsats, Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

   Författare :Eleni Pechlivani; [2023]
   Nyckelord :1; 3-Propanediol; Glycerol dehydratase; 3-Propanediol dehydrogenase; Glycerol dehydratase reactivase; E. coli; Protein expression; Biotechnology; Technology and Engineering;

   Sammanfattning : The biodiesel sector has witnessed tremendous growth in recent years, leading to a significant increase in glycerol production as a by-product. Addressing the challenge of glycerol valorization is crucial for the sustainability of the biodiesel industry. LÄS MER

3. 2. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

   Master-uppsats, KTH/Tillämpad fysik

   Författare :Emma Westlund; [2023]
   Nyckelord :ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

   Sammanfattning : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. LÄS MER

4. 3. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

   Master-uppsats, Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

   Författare :Erik Apelqvist; [2022]
   Nyckelord :Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

   Sammanfattning : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. LÄS MER

5. 4. The transfer of chromosomal genes through bacterial conjugation in Escherichia coli

   Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Författare :Arijana Katana; [2021]
   Nyckelord :conjugation; escherichia coli; hybrid;

   Sammanfattning : Evolution in bacteria occurs through the combined effects of spontaneous mutations and horizontal gene transfer (HGT). Several mechanisms can lead to HGT: (i) transformation, the uptake of DNA from the environment; (ii) transduction, the transfer of DNA carried by a bacteriophage into another bacterium during infection; and (iii) conjugation, bacterial mating mediated by a conjugative plasmid like the F-factor. LÄS MER

7. 5. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

   Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

   Författare :Alexander Westlund; [2019]
   Nyckelord :Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

   Sammanfattning : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. LÄS MER