Sökning: "fosfatbuffert"

Hittade 5 uppsatser innehållade ordet fosfatbuffert.

  1. 1. Exploring alternative surfactants to replace PEG within lipid nanoparticles

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master); Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Författare :Elina Edidovica; [2024]
    Nyckelord :Lipid nanoparticle; polyethelyne glycol PEG ; alpha-tomatine; N-hexadecyl beta d-maltoside HDM ; DOPC; DPPC; cholesterol; film hydration; stability study; DLS; Cryo-TEM; SAXS; pharmaceutical formulation; Chemistry;

    Sammanfattning : Over the past decade an increasing amount of studies has demonstrated that PEGylation not only precipitates reductions in drug delivery efficiency but also triggers the immune system to produce antibodies, specifically anti-PEG antibodies. The objective of my thesis is to explore alternative surfactants that could viably replace PEG within lipid nanoparticles. LÄS MER

  3. 2. Optimising microfluidic preparation of lipid sponge phase nanoparticles for biomolecule encapsulation

    Master-uppsats, Lunds universitet/Kemiteknik (CI)

    Författare :Owais Khalil; [2023]
    Nyckelord :Sponge phase; lipid nanoparticles; microfluidics; SAXS; cryo-TEM; DLS; chemical engineering; Chemistry;

    Sammanfattning : Drug delivery with lipid nanoparticles, especially sponge phase lipid nanoparticles (LNP), has been well studied because of its ability for drug encapsulation and transferring the drug through the cell membrane. The sponge phase LNP formulation could be affected by the molecular structure of the lipid, the temperature, pH, dilution, the buffer, and the addition of a stabiliser. LÄS MER

  4. 3. Protein environment and structure in neat and hydrated deep eutectic solvents

    Master-uppsats, Lunds universitet/Livsmedelsteknik och nutrition (master)

    Författare :Jenny Xiang; Medina Basic; [2020]
    Nyckelord :Deep eutectic solvents; proteins; BSA; lysozyme; choline chloride:glycerol; choline chloride:urea; choline acetate:glycerol; UV-Vis; fluorescence; FT-IR; hydrated deep eutectic solvents; pharmaceutical technology; läkemedelsteknologi; Technology and Engineering;

    Sammanfattning : The use of deep eutectic solvents (DESs) has a great potential in the pharmaceutical field due to its many advantages such as biocompatibility, low toxicity and biodegradability. Studies have also shown that proteins can be successfully incorporated into DESs. LÄS MER

  5. 4. Development of Wet Electrostatic Precipitator for Generation of Nanoparticle-Protein Solution

    Master-uppsats, Lunds universitet/Ergonomi och aerosolteknologi

    Författare :Julia Adner; [2015]
    Nyckelord :WESP; Nanoparticle; bioligical identity; protein solution; Technology and Engineering;

    Sammanfattning : To examine nanoparticle toxicity there is a need to produce defined nanoparticles dispersed in a colloidal suspension. Such a solution could also be interesting for other fields such as nano-medicine. In order to disperse the particles in the liquid there are often surfactant added to the process that alternates the surface of the particles. LÄS MER

  7. 5. Hur påverkas CellaVision DM1200:s förklassificering av leukocyternas infärgning?

    Kandidat-uppsats, Högskolan Kristianstad/Sektionen för lärande och miljö

    Författare :Lina Andersson; [2015]
    Nyckelord :Leukocyter; neutrofila granulocyter; eosinofila granulocyter; basofila granulocyter; monocyter; lymfocyter; omogna celler; automatiserade cellräknare; May Grünwald-Giema; Romanowsky; fosfatbuffert; differentialräkning; CellaVision DM1200;

    Sammanfattning : Sammansättningen av leukocyter i perifert blod varierar med olika sjukdomstillstånd och analys av cellerna är avgörande vid diagnostisering och uppföljning av olika leukemier.  Manuell mikroskopering och utvärdering av leukocyterna är tidskrävande och inte alltid självklar eftersom det krävs personal med hög kompetens och erfarenhet för bedömning av cellerna. LÄS MER