Sökning: "gene engineering"

Visar resultat 1 - 5 av 56 uppsatser innehållade orden gene engineering.

  1. 1. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis

    Master-uppsats, Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Författare :Pennie Lindblom; Olivia Geel; [2023]
    Nyckelord :Phytoglobin; Heme; δ-ALA; qPCR; SDS-PAGE; Bacterial cultivation; Escherichia coli; BvPgb1.2; ÄKTA; Applied biochemistry; Biology and Life Sciences;

    Sammanfattning : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. LÄS MER

  3. 2. How molecular diagnostics based on gene expressions can improve diagnostics in Sarcoma

    Kandidat-uppsats, Lunds universitet/Avdelningen för Biomedicinsk teknik

    Författare :Emma Friberg; Alma Lennartsson; [2023]
    Nyckelord :Technology and Engineering;

    Sammanfattning : Sarcoma, a rare and heterogeneous cancer type, present significant diagnostic challenges due to its numerous subtypes. Molecular diagnostics and machine learning models have emerged as promising tools to enhance sarcoma diagnosis. LÄS MER

  4. 3. Toward prototyping metabolic pathways in cyanobacteria using cell extracts

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Amina Bensabra; [2022]
    Nyckelord :cyanobacteria; metabolic engineering; cell-free metabolic engineering; enzymes; sustainability; cyanobakterier; metabolisk genteknik; syntetisk biologi; enzymer; hållbar bioproduktion;

    Sammanfattning : Cyanobakterier är intressanta mikroorganismer för produktion av biobränslen från solljus, vatten och atmosfärisk koldioxid och anses därför vara potentiella mikrobiella cellfabriker. Men på grund av långsam tillväxt och låg produktion är genteknologi processen intensiv och tidskrävande för cyanobakterier. LÄS MER

  5. 4. Evaluation of strategies for construction of protease libraries in yeast.

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Författare :Sidharth Jaya Sankar; [2022]
    Nyckelord :Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Sammanfattning : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. LÄS MER

  7. 5. Genetically modified tobacco (Nicotiana tabacum) plants for an increased production of wax esters

    Kandidat-uppsats, SLU/Dept. Of Plant Biology

    Författare :Linn Nilsson; [2022]
    Nyckelord :Wax esters; metabolic engineering; nicotiana tabacum; fatty acid reductase; phytyl ester synthase;

    Sammanfattning : Wax esters (WE) are naturally occurring lipids consisting of a fatty acid bound to a fatty alcohol with an ester bond. As an alternative to petrochemicals, plant-derived WE:s are of considerable commercial interest as lubricants, but have in recent years also received great attention for their potential as a starting material for bio-fuel production. LÄS MER