Sökning: "genetic manipulation"

Visar resultat 1 - 5 av 12 uppsatser innehållade orden genetic manipulation.

  1. 1. Genetic manipulation to improve efficacy of dendric cell adoptive immunotherapy against cancer in dogs

    Kandidat-uppsats, Karlstads universitet

    Författare :Felicia Berglund; [2023]
    Nyckelord :Adoptive immunotherapi;

    Sammanfattning : To improve the efficacy of the dendritic cell vaccine Alv B DC from Alv B, the PD-L1 expression in cancer cells was attempted to be reduced through a transfection with a custom designed siRNA. Before transfecting the dendritic cells, the siRNA functionality had to be tested through flow cytometry, that resulted in negative results and therefore led to a RT-qPCR protocol that indicated that the siRNA was functional. LÄS MER

  3. 2. Evaluation of strategies for construction of protease libraries in yeast.

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Författare :Sidharth Jaya Sankar; [2022]
    Nyckelord :Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Sammanfattning : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. LÄS MER

  4. 3. Assessment of promoter regulation in Pseudomonas putida using GFP and flow cytometry

    Master-uppsats, Lunds universitet/Teknisk mikrobiologi

    Författare :Luka Cululejevic; [2022]
    Nyckelord :applied microbiology; Pseudomonas putida; promoter characterization; aromatic compounds; vanillin; guaiacol; Biology and Life Sciences;

    Sammanfattning : Lignin is found in all biomass and is an abundant natural source of aromatic compounds. It has the potential to serve as a renewable raw material for a variety of chemicals currently derived from fossil fuel sources such as muconic acid, an important platform chemical. Kraft lignin is a waste stream from the pulp and paper industry. LÄS MER

  5. 4. Fekunditet hos honor av Drosophila melanogaster med en potentiell sexuellt antagonistisk gen : En fördjupande studie inom experimentell validering av en potentiell sexuellt antagonistisk gen hos Drosophila melanogaster

    Kandidat-uppsats, Karlstads universitet/Institutionen för miljö- och livsvetenskaper (from 2013)

    Författare :Sara Lindh; [2022]
    Nyckelord :sexual antagonism; sexual conflicts; fruitflies; drosophila melanogaster; drosophila; GWAS; antagonistic sexual selection; fecundity; intralocus sexual conflict; reproductive fitness; sexuell konflikt; sexuella konflikter; drosophila melanogaster; drosophila; bananflugor; sexuell antagonism; fekunditet;

    Sammanfattning : Sexual conflicts arise when there is a difference in how females and males of a species or population achieve their maximum reproductive fitness. In intralocus sexual conflicts, alleles at a given locus are exposed to conflicting, or antagonistic, selection pressures. LÄS MER

  7. 5. Designing an optmized met X/Y enzymes for an efficient methionine production in E.coli

    Kandidat-uppsats, Högskolan i Skövde/Institutionen för biovetenskap

    Författare :Luísa Rodrigues; [2022]
    Nyckelord :;

    Sammanfattning : Methionine is a proteinogenic amino acid critical amino for the pharmaceutical, biotechnological and agricultural industries. The overall methionine biosynthetic pathway is as follows: 1. Acetylation of homoserine and its subsequent activation 2. Formation of homoserine through the replacement hydroxyl group by a thiol group, 3. LÄS MER