Sökning: "glycobiology"

Hittade 1 uppsats innehållade ordet glycobiology.

  1. 1. Expression of the heparan sulfate biosynthesis enzymes NDST1 and NDST2 and their major splice variants in human tissues.

    L3-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Fredrik Kristoffersson; [2018]
    Nyckelord :Heparan sulfate; NDST; glycobiology; N-Deacetylase And N-Sulfotransferase; NGS; bioinformatics;

    Sammanfattning : The aim of the study was to investigate the expression NDST transcripts in a wide variety of tissues using RNA-sequencing experimental data from five published studies, using two common in silico tools: the Tophat-Cufflink pipeline and the HTSeq-DEXSeq pipeline. We show that to detect NDST alternative transcripts, paired-end sequencing should be used with replicates of samples or conditions together with 100 base read length to allow for reliable detection of the low expressed transcripts in the NDST family. LÄS MER