Sökning: "histidine-tagged enzyme"

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  1. 1. Biosensor based on immobilized amine transaminase for detection of amphetamine

    Master-uppsats, KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Författare :Clara Öh; [2020]
    Nyckelord :amine transaminases ATA ; enzyme immobilization; biosensor; covalent immobilization; ionic immobilization; amphetamine; histidine-tagged enzyme; Poly methyl methacrylate PMMA ;

    Sammanfattning : Amine transaminases (ATA) catalyse the transfer of an amino group from one molecule and replaces a ketone or aldehyde with the amino group, the amino group on the amino-donor is replaced with a ketone or aldehyde. This enzyme, ATA from Chromobacterium violaceum, has previously been used to catalyse the reaction involving amphetamine, therefore, it might be possible to use this enzyme to convert amphetamine and the product absorbs in the UV spectrum and can therefore be measured spectrophotometrically. LÄS MER