Sökning: "human recombinant antibodies"

Visar resultat 1 - 5 av 12 uppsatser innehållade orden human recombinant antibodies.

  1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

    Master-uppsats, Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Författare :Basel Yousef; [2024]
    Nyckelord :;

    Sammanfattning : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. LÄS MER

  3. 2. Optimization of Calcium-Dependent Affinity Ligands for Protein Purification

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Linnea Öst; [2021]
    Nyckelord :Protein purification; Protein A; calcium-dependent; scFv; G-CSF; multimerization; mild elution; ZCa;

    Sammanfattning : With an expanding life-science sector and growing production of recombinant proteins, the need for efficient downstream processing is increasing. Certain proteins are sensitive to the harsh conditions often used in protein purification, such as low pH, which can result in aggregation and denaturation. LÄS MER

  4. 3. Development of a high-throughput platform for generation and early screening of high producing stable cell lines

    Master-uppsats, KTH/Proteinvetenskap

    Författare :Jonathan Karlsson Persson; [2021]
    Nyckelord :CHO-K1; HEK293; CellCelector™; High-throughput screening; Protein A beads; Secondary antibody; CHO-K1; HEK293; CellCelector™; Högkapacitets-screening; Protein A-kulor; Sekundär antikropp;

    Sammanfattning : Produktionen av rekombinanta biofarmaceutiska läkemedel, exempelvis monoklonala antikroppar, är en oumbärlig men ansträngande process. Under 2019 var sju av de tio mest sålda läkemedlen globalt baserade på rekombinant producerade monoklonala antikroppar. LÄS MER

  5. 4. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    Master-uppsats, Linköpings universitet/Institutionen för fysik, kemi och biologi

    Författare :Gustav Myhrinder; [2020]
    Nyckelord :Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Sammanfattning : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. LÄS MER

  7. 5. Development and comparison of three immunoassay formats to screen for total anti-adeno-associated virus serotype 2 antibodies in human serum using the Gyrolab immunoassay platform

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Elin Eriksson; [2020]
    Nyckelord :Adeno-associated virus; AAV; anti-AAV; TAb; Gyrolab; immunity; immunoassay;

    Sammanfattning : Recombinant adeno-associated virus vectors are one of the most promising gene delivery tools for applications within gene- and cell therapy. The high level of wild-type adeno-associated virus infections in humans is a limitation due to the pre-existing immunity against the vector or its transgene product. LÄS MER