Sökning: "hydrophobic interaction"

Visar resultat 1 - 5 av 23 uppsatser innehållade orden hydrophobic interaction.

  1. 1. Screening of HETP test conditions and resin storage solutions for HIC

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Analytisk farmaceutisk kemi

    Författare :Lise-Lotte Ronnerfors; [2023]
    Nyckelord :Hydrophobic Interaction Chromatography; Pfizer Health AB; Column packing;

    Sammanfattning : Hydrophobic interaction chromatography (HIC) is a common purification method for biological drug substances. For continuous monitoring the quality of the purification, a column performance test is carried out to test the packing of the resin. LÄS MER

  3. 2. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    Kandidat-uppsats, Uppsala universitet/Institutionen för biologisk grundutbildning

    Författare :Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Nyckelord :Plasmid purification; pDNA; large-scale chromatography;

    Sammanfattning : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. LÄS MER

  4. 3. Comparison and optimization of extracellular vesicle (EV) capturing on functional thin films for their molecular profiling

    Master-uppsats, KTH/Tillämpad fysik

    Författare :Prattakorn Metem; [2023]
    Nyckelord :extracellular vesicle; plasmonic nanohole array; atomic force microscopy; fluorescence analysis; extracellulära vesiklar; nanohålsuppsättning; atomkraftsmikroskopi; fluorescensanalys;

    Sammanfattning : Extracellular vesicles (EVs) are lipid bilayer encapsulated nanoparticles which have emerged as an excellent source of biomarkers for multiple diseases, including cancer. However, they are highly heterogeneous in their molecular compositions which remains a major challenge hindering the utilization of their biomarker potential. LÄS MER

  5. 4. Design, synthesis, and evaluation of 1-phenylethanol and C2 threitol derivatives as non-pyranose galactose-mimicking inhibitors of Galectin-3

    Master-uppsats, Lunds universitet/Centrum för analys och syntes

    Författare :Qrystal Cruz Giron; [2023]
    Nyckelord :Galectin-3; Galectin-3 inhibitor; 1-phenylethanol; C2 threitol derivative; galactose-mimicking inhibitor; organic chemistry; Chemistry;

    Sammanfattning : Galectins are cytosolic protein family involved in several biological functions such as cell differentiation, cell migration and apoptosis amongst many others. Their presence is also substantial in diseases including acute inflammation, fibrosis, allergies, and cancer. LÄS MER

  7. 5. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    Uppsats för yrkesexamina på avancerad nivå, Uppsala universitet/Biokemi

    Författare :Sofia Larsson; [2023]
    Nyckelord :;

    Sammanfattning : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. LÄS MER